Development and validation of a rapid RP-HPLC method for analysis of (-)-copalic acid in copaiba oleoresin

Author(s)

Souza, Ariana Borges ^[1] ; Moreira, Monique Rodrigues ^[1] ; Gambeta Borges, Carly Henrique ^[1] ; Simao, Marilia Rodrigues ^[1] ; Bastos, Jairo Kenupp ^[2] ; Barreto de Sousa, Joao Paulo ^[2] ; Ambrosio, Sergio Ricardo ^[1] ; Sola Veneziani, Rodrigo Cassio ^[1]

Affiliation

^[1] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, BR-14404600 Franca, SP - Brazil

^[2] Univ Sao Paulo, Lab Farmacognosia, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: BIOMEDICAL CHROMATOGRAPHY; v. 27, n. 3, p. 280-283, MAR 2013.
• Web of Science Citations: 10

Abstract

The Copaifera species (Leguminoseae) are popularly known as copaiba' or copaiva' and are grown in the states of Amazonas, Para and Ceara in northern Brazil. The oleoresins obtained from these species have been extensively used owing to their pharmacological potential and their application in cosmetic and pharmaceutical preparations. In the present study, the development and validation of a novel, rapid and efficient RP-HPLC methodology for the analysis of the diterpene ()-copalic acid (CA), pointed out as the only chemical marker of the Copaifera genus, are described. The regression equation (Y=26,707x29,498) was obtained with good linearity (r2=0.9993) and the limits of quantification and detection were 9.182 and 3.032 mu g/mL, respectively. The precision and the accuracy of the method were adequate (lower than 4%). Finally, the validation parameters evaluated were satisfactorily met, so the developed method represents a suitable tool for application in the quality control of such natural products. Further studies aiming to develop analytical methodologies for each Copaifera species using a more representative number of chemical markers should be performed. Copyright (c) 2012 John Wiley \& Sons, Ltd. (AU)

• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/12796-9 - Copaifera langsdorfii oleoresin: isolation of secondary metabolites, development and validation of analytical methodology by HPLC
• Grantee: Ariana Borges Souza
• Support Opportunities: Scholarships in Brazil - Master