Differential Response of Heat Shock Proteins to Uphill and Downhill Exercise in Heart, Skeletal Muscle, Lung and Kidney Tissues

Author(s)

Lollo, Pablo C. B. ^[1] ; Moura, Carolina S. ^[1] ; Morato, Priscila N. ^[1] ; Amaya-Farfan, Jaime ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Food & Nutr, Sch Food Engn, UNICAMP, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF SPORTS SCIENCE AND MEDICINE; v. 12, n. 3, p. 461-466, SEP 2013.
• Web of Science Citations: 20

Abstract

Running on a horizontal plane is known to increase the concentration of the stress biomarker heat-shock protein (HSP), but no comparison of the expression of HSP70 has yet been established between the uphill (predominantly concentric) and downhill (predominantly eccentric) muscle contractions exercise. The objective of the study was to investigate the relationships between eccentric and concentric contractions on the HSP70 response of the lung, kidney, gastrocnemius, soleus and heart. Twenty-four male Wistar weanling rats were divided into four groups: non-exercised and three different grades of treadmill exercise groups: horizontal, uphill (+7%) and downhill (-7% of inclination). At the optimal time-point of six hours after the exercise, serum uric acid, creatine kinase (CK) and lactate dehydrogenase (LDH) were determined by standard methods and HSP70 by the Western blot analysis. HSP70 responds differently to different types of running. For kidney, heart, soleus and gastrocnemius, the HSP70 expression increased, 230, 180, 150 and 120% respectively of the reference (horizontal). When the contraction was concentric (uphill) and compared to downhill the increase in response of HSP70 was greater in 80% for kidney, 75% for gastrocnemius, 60% for soleus and 280% for the heart. Uric acid was about 50% higher (0.64+-/ 0.03 mg.dL(-1)) in the uphill group as compared to the horizontal or downhill groups. Similarly, the activities of serum CK and LDH were both 100% greater for both the uphill and downhill groups as compared to the horizontal group (2383+-/ 253 and 647.00+-/ 73 U/L, respectively). The responsiveness of HSP70 appeared to be quite different depending on the type of tissue, suggesting that the impact of exercise was not restricted to the muscles, but extended to the kidney tissue. The uphill exercise increases HSP70 beyond the eccentric type and the horizontal running was a lower HSP70 responsive stimulus. (AU)

• FAPESP's process: 11/13035-1 - The effect of consumption of milk protein serums on HSP biomarkers and biochemical parameters in rats
• Grantee: Jaime Amaya Farfan
• Support Opportunities: Regular Research Grants