| Full text | |
| Author(s): |
Machado, Susane Moreira
[1]
;
Pacheco-Soares, Cristina
[1, 2]
;
Marciano, Fernanda Roberta
[3]
;
Lobo, Anderson Oliveira
[3]
;
da Silva, Newton Soares
[1, 2]
Total Authors: 5
|
| Affiliation: | [1] Univ Vale Paraiba UNIVAP, Dev & Res Inst IP&D, Lab Tissue & Cell Biol, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Vale Paraiba UNIVAP, Dev & Res Inst IP&D, Lab Dynam Cellular Compartments, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[3] Univ Vale Paraiba UNIVAP, Dev & Res Inst IP&D, Lab Biomed Nanotechnol, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Materials Science & Engineering C-Materials for Biological Applications; v. 36, p. 180-186, MAR 1 2014. |
| Web of Science Citations: | 5 |
| Abstract | |
Superhydrophilic vertically aligned carbon nanotubes (VACNT-O-2) were used for the first time as scaffolds for photodynamic therapy (PDT) to induce inhibition of cell division in eukaryotic cells. VACNT-O-2 scaffolds were produced on Ti substrates using plasma enhanced chemical vapor deposition technique and functionalized by oxygen plasma. Scanning electron microscopy (SEM) analysis was performed to characterize the surface changes of the protozoan and interaction with VACNT-O-2. Characterization of lipid and total protein expression was performed with protozoa that were or not treated with PDT. Quantification of protein was conducted using Qubit fluorometer and separated on a polyacrylamide gel. SEM analysis showed the release of lipid vesicles by protozoa after the PDT. These vesicles were characterized by the PKH26 fluorescent probe. The results demonstrated a greater amount of protein released after PDT than in the control. When analyzing the protein material in polyacrylamide gel, a significant protein expression of approximately 65 kDa was found. A model identified the programmed death of Tritrichomonas foetus after the PDT was also proposed. (C) 2013 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications |
| Grantee: | Fernanda Roberta Marciano |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 08/06654-4 - Photodynamic therapy and cell death activation in the cattle parasitic protozoan Tritrichomonas foetus |
| Grantee: | Newton Soares da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration |
| Grantee: | Anderson de Oliveira Lobo |
| Support Opportunities: | Research Grants - Young Investigators Grants |