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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial Evaluation of Traumatized Teeth Treated with Triple Antibiotic Paste or Calcium Hydroxide with 2% Chlorhexidine Gel in Pulp Revascularization

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Author(s):
Nagata, Juliana Y. [1] ; Soares, Adriana J. [1] ; Souza-Filho, Francisco J. [1] ; Zaia, Alexandre A. [1] ; Ferraz, Caio C. R. [1] ; Almeida, Jose F. A. [1] ; Gomes, Brenda P. F. A. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Dept Restorat Dent, Endodont Div, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 40, n. 6, p. 778-783, JUN 2014.
Web of Science Citations: 29
Abstract

Introduction: Revascularization outcome depends on microbial elimination because apical repair will not happen in the presence of infected tissues. This study evaluated the microbial composition of traumatized immature teeth and assessed their reduction during different stages of the revascularization procedures performed with 2 intracanal medicaments. Methods: Fifteen patients (7-17 years old) with immature teeth were submitted to the revascularization procedures; they were divided into 2 groups according to the intracanal medicament used: TAP group (n = 7), medicated with a triple antibiotic paste, and CHP group (n = 8), dressed with calcium hydroxide + 2% chlorhexidine gel. Samples were taken before any treatment (S1), after irrigation with 6% NaOCl (S2), after irrigation with 2% chlorhexidine (S3), after intracanal dressing (S4), and after 17% EDTA irrigation (S5). Cultivable bacteria recovered from the 5 stages were counted and identified by means of polymerase chain reaction assay (165 rRNA). Results: Both groups had colony-forming unit counts significantly reduced after S2 (P < .05); however, no significant difference was found between the irrigants (S2 and S3, P = .99). No difference in bacteria counts was found between the intracanal medicaments used (P = .95). The most prevalent bacteria detected were Actinomyces naeslundii (66.67%), followed by Porphyromonas endodontalis, Parvimonas micra, and Fusobacterium nucleatum, which were detected in 33.34% of the root canals. An average of 2.13 species per canal was found, and no statistical correlation was observed between bacterial species and clinical/radiographic features. Conclusions: The microbial profile of infected immature teeth is similar to that of primarily infected permanent teeth. The greatest bacterial reduction was promoted by the irrigation solutions. The revascularization protocols that used the tested intracanal medicaments were efficient in reducing viable bacteria in necrotic immature teeth. (AU)

FAPESP's process: 11/09047-4 - Investigation of microbial diversity from primary endodontic infection by clonal analysis and 16S rRNA sequencing
Grantee:Brenda Paula Figueiredo de Almeida Gomes
Support Opportunities: Regular Research Grants