| Full text | |
| Author(s): Show less - |
Ribeiro, Liliane F. C.
[1]
;
De Lucas, Rosymar C.
[1]
;
Vitcosque, Gabriela L.
[1]
;
Ribeiro, Lucas F.
[1]
;
Ward, Richard J.
[2, 3]
;
Rubio, Marcelo V.
[2]
;
Damasio, Andre R. L.
[2]
;
Squina, Fabio M.
[2]
;
Gregory, Rebecca C.
[4]
;
Walton, Paul H.
[4]
;
Jorge, Joao A.
[5]
;
Prade, Rolf A.
[6]
;
Buckeridge, Marcos S.
[7]
;
Polizeli, Maria de Lourdes T. M.
[5]
Total Authors: 14
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Immunol & Biochem Dept, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Campinas, SP - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Chem, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Univ York, Dept Chem, York YO10 5DD, N Yorkshire - England
[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil
[6] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA
[7] Univ Sao Paulo, Inst Biosci, Sao Paulo - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | BIOTECHNOLOGY FOR BIOFUELS; v. 7, JUL 29 2014. |
| Web of Science Citations: | 28 |
| Abstract | |
Background: The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic fungus Malbranchea pulchella, the influence of glycosylation on its stability, and a potential application in sugarcane bagasse hydrolysis. Results: Xylanase MpXyn10A was overexpressed in Aspergillus nidulans and was active against birchwood xylan, presenting an optimum activity at pH 5.8 and 80 degrees C. MpXyn10A was 16% glycosylated and thermostable, preserving 85% activity after 24 hours at 65 degrees C, and deglycosylation did not affect thermostability. Circular dichroism confirmed the high alpha-helical content consistent with the canonical GH10 family (beta/alpha)(8) barrel fold observed in molecular modeling. Primary structure analysis revealed the existence of eight cysteine residues which could be involved in four disulfide bonds, and this could explain the high thermostability of this enzyme even in the deglycosylated form. MpXyn10A showed promising results in biomass degradation, increasing the amount of reducing sugars in bagasse in natura and in three pretreated sugarcane bagasses. Conclusions: MpXyn10A was successfully secreted in Aspergillus nidulans, and a potential use for sugarcane bagasse biomass degradation was demonstrated. (AU) | |
| FAPESP's process: | 10/18850-2 - Identification, characterization and engineering of plant cell wall degrading enzymes |
| Grantee: | Richard John Ward |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/12859-3 - Functional study of recombinant GH12 xiloglucanases for xyloglucan hydrolysis |
| Grantee: | Marcelo Ventura Rubio |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 13/18910-3 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases |
| Grantee: | André Ricardo de Lima Damasio |
| Support Opportunities: | Scholarships in Brazil - BIOEN - Young Researchers |
| FAPESP's process: | 10/07133-8 - Creation of an Allosteric Endoxylanase by Directed Evolution for application in Industrial Biotechnology |
| Grantee: | Lucas Ferreira Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 10/10184-3 - Development of an enzymatic cocktail for application in biorefinery |
| Grantee: | Liliane Fraga Costa Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/02017-5 - Production of recombinant beta-xylosidase for application on bioethanol production line |
| Grantee: | Gabriela Leal Vitcosque |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 08/57908-6 - National Institute of Science and Technology of Bioethanol |
| Grantee: | Marcos Silveira Buckeridge |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |