Understanding the molecular biology and ecology of plant-virus-vector relationships: towards sustainable, integrated virus management strategies

• Grant number: 17/50222-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2018
• End date: May 31, 2022
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Agreement: Netherlands Organisation for Scientific Research (NWO)
• Principal Investigator: Juliana de Freitas Astúa
• Grantee: Juliana de Freitas Astúa
• Principal researcher abroad: Richard Jozef Maria Kormelink
• Institution abroad: Wageningen University, Netherlands
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Pathogens present one of the major constraints on production yields and with nearly 50% of all newly emerging diseases caused by viruses, stresses the importance for continuing development of integrated virus management (IVM) strategies. The 1) emergence (2016) of new recombinant geminivirus in cultivations of tomato bearing the Ty-1 resistance gene; 2) observation (2014) that mixed infections of TYLCV and CMV compromises Ty-1- mediated resistance against geminiviruses; 3) identification (unpublished) of TSWV isolates able to overcome Tsw resistance gene in pepper; 4) new Bemisia tabaci biotype (unpublished) establishing on pepper and able to more efficiently transmit certain geminiviruses; 5) new dichorhavirus species (2017) and isolates of citrus leprosis cilevirus C (2016) causing leprosis disease in Brazil; 6) reclassification of genus Brevipalpus into several new vector species (2015), are just a few examples that underscore the importance of continuing multidiscipline research efforts to study and unravel the molecular biology and ecology of plant-virus-vector relationships. This project aims to strengthen joint efforts of a Dutch-Brazilian virology research consortium to study a small set of very important plant viruses to the Dutch and/or Brazilian Agri-Food sector in different vegetable and fruit crops and transmitted by various arthropods. This project builds on an earlier NWO-CPNq supported project and expands towards the establishment of a larger collaborating research network that will not only speed up the implementation of newly gained knowledge into sustainable IVM approaches for the viruses under investigation but is likely to be of use to other virus pathosystems as well, and meantime presents an international research platform to maintain plant virology expertise needed for exchange and training plant virology students. (AU)

Scientific publications

(24)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMOS-GONZALEZ, PEDRO LUIS; DA COSTA-RODRIGUES, MARIANE; POTSCLAM-BARRO, MATHEUS; CHABI-JESUS, CAMILA; BANGUELA-CASTILLO, ALEXANDER; HARAKAVA, RICARDO; KITAJIMA, ELLIOT W.; FREITAS-ASTUA, JULIANA. First genome sequence of an isolate of hibiscus chlorotic ringspot virus from the Western hemisphere. TROPICAL PLANT PATHOLOGY, v. 45, n. 2, FEB 2020. (14/08458-9, 17/50222-0, 17/50334-3, 19/09222-2, 16/01960-6)

TASSI, ALINE D.; DUARTE, ADRIANE F.; DA CUNHA, UEMERSON S.; OCHOA, RONALD. First record of family Linotetranidae in Brazil, with a redescription of Linotetranus achrous Baker and Pritchard and description of a new species. INTERNATIONAL JOURNAL OF ACAROLOGY, v. 46, n. 8, p. 579-588, NOV 16 2020. (14/08458-9, 18/12252-8, 17/50222-0)

GORAYEB, EDUARDO SILVA; WATANABE, LUIS FERNANDO MARANHO; PEREIRA, YAGO ALEXANDRE BARBI; DOVIGO, LEONARDO HIPOLITO; BELLO, VINICIUS HENRIQUE; SOUZA, ISABELA MORCILO DE; CRUCIOL, GIOVANA CAROLINA DOURADO; VICENTIN, EDUARDO; SARTORI, MARIA MARCIA PEREIRA; KRAUSE-SAKATE, RENATE. Performance and preference of Bemisia tabaci on cucumbers: Understanding the recent outbreaks of Mediterranean cryptic species in Brazil. CROP PROTECTION, v. 143, MAY 2021. (17/50222-0, 19/08377-2, 17/21588-7, 18/18274-3)

DIETZGEN, RALF G.; BEJERMAN, NICOLAS E.; MEI, YONGYU; JEE, CHARMAINE LIM JING; CHABI-JESUS, CAMILA; FREITAS-ASTUA, JULIANA; VERAS, SOLANGE M.; KITAJIMA, ELLIOT W.. Joa yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil. ARCHIVES OF VIROLOGY, v. 166, n. 6, MAR 2021. (17/50222-0, 17/18910-4)

RAMOS-GONZALEZ, PEDRO L.; PONS, TIRSO; CHABI-JESUS, CAMILA; ARENA, GABRIELLA DIAS; FREITAS-ASTUA, JULIANA. oorly Conserved P15 Proteins of Cileviruses Retain Elements of Common Ancestry and Putative Functionality: A Theoretical Assessment on the Evolution of Cilevirus Genome. FRONTIERS IN PLANT SCIENCE, v. 12, NOV 5 2021. (19/02137-0, 19/25078-9, 17/50222-0, 20/15413-2)

ARENA, GABRIELLA D.; RAMOS-GONZALEZ, PEDRO L.; ROGERIO, LUANA A.; RIBEIRO-ALVES, MARCELO; CASTEEL, CLARE L.; FREITAS-ASTUA, JULIANA; MACHADO, MARCOS A.. Making a Better Home: Modulation of Plant Defensive Response by Brevipalpus Mites. FRONTIERS IN PLANT SCIENCE, v. 9, AUG 15 2018. (17/50222-0, 14/50880-0, 14/00366-8, 16/23870-9)

ARENA, GABRIELLA D.; RAMOS-GONZALEZ, PEDRO LUIS; FALK, BRYCE W.; CASTEEL, CLARE L.; FREITAS-ASTUA, JULIANA; MACHADO, MARCOS A.. Plant Immune System Activation Upon Citrus Leprosis Virus C Infection Is Mimicked by the Ectopic Expression of the P61 Viral Protein. FRONTIERS IN PLANT SCIENCE, v. 11, AUG 7 2020. (16/23870-9, 17/50222-0, 14/00366-8, 19/02137-0, 14/50880-0)

CHABI-JESUS, CAMILA; RAMOS-GONZALEZ, PEDRO LUIS; TASSI, ALINE DANIELE; ROSSETTO PEREIRA, LAURA; BASTIANEL, MARINES; LAU, DOUGLAS; CANALE, MARIA CRISTINA; HARAKAVA, RICARDO; NOVELLI, VALDENICE MOREIRA; KITAJIMA, ELLIOT WATANABE; et al. Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. PLANTS-BASEL, v. 12, n. 6, p. 15-pg., 2023-03-01. (17/50222-0, 20/15413-2, 19/02137-0, 18/12252-8, 17/50334-3, 19/25078-9)

LEASTRO, MIKHAIL OLIVEIRA; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS ANGEL. Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins. FRONTIERS IN MICROBIOLOGY, v. 11, NOV 4 2020. (17/19898-8, 15/10249-1, 17/50222-0)

LEASTRO, MIKHAIL OLIVEIRA; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS A.. Unravelling the involvement of cilevirus p32 protein in the viral transport. SCIENTIFIC REPORTS, v. 11, n. 1, FEB 3 2021. (15/10249-1, 17/50222-0, 17/19898-8)

LEASTRO, MIKHAIL OLIVEIRA; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS A.. Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins. MICROORGANISMS, v. 9, n. 2, FEB 2021. (15/10249-1, 17/19898-8, 17/50222-0)

RAMOS-GONZALEZ, PEDRO LUIS; KONDO, HIDEKI; MOROZOV, SERGEY; VASILAKIS, NIKOLAOS; VARSANI, ARVIND; CAO, MENGJI; FREITAS-ASTUA, JULIANA. Editorial: The Border Between Kitavirids and Nege-Like Viruses: Tracking the Evolutionary Pace of Plant- and Arthropod-Infecting Viruses. FRONTIERS IN PLANT SCIENCE, v. 13, p. 2-pg., 2022-05-24. (17/50334-3, 19/25078-9, 17/50222-0)

RAMOS-GONZALEZ, PEDRO LUIS; DA COSTA-RODRIGUES, MARIANE; POTSCLAM-BARRO, MATHEUS; CHABI-JESUS, CAMILA; BANGUELA-CASTILLO, ALEXANDER; HARAKAVA, RICARDO; KITAJIMA, ELLIOT W.; FREITAS-ASTUA, JULIANA. First genome sequence of an isolate of hibiscus chlorotic ringspot virus from the Western hemisphere. ROPICAL PLANT PATHOLOG, v. 45, n. 2, p. 6-pg., 2020-02-05. (17/50334-3, 14/08458-9, 17/50222-0, 16/01960-6, 19/09222-2)

CHABI-JESUS, C.; RAMOS-GONZALEZ, P. L.; TASSI, A. D.; BARGUIL, B. M.; BESERRA JUNIOR, J. E. A.; HARAKAVA, R.; KITAJIMA, E. W.; FREITAS-ASTUA, J.. First Report of Citrus Chlorotic Spot Virus Infecting the Succulent Plant Agave desmettiana. PLANT DISEASE, v. 103, n. 6, p. 1-pg., 2019-06-01. (16/01960-6, 17/50334-3, 14/08458-9, 17/50222-0)

RAMOS-GONZALEZ, PEDRO LUIS; DOS SANTOS, GUSTAVO FRANCISCO; CHABI-JESUS, CAMILA; HARAKAVA, RICARDO; KITAJIMA, ELLIOT W.; FREITAS-ASTUA, JULIANA. Passion Fruit Green Spot Virus Genome Harbors a New Orphan ORF and Highlights the Flexibility of the 5 `-End of the RNA2 Segment Across Cileviruses. FRONTIERS IN MICROBIOLOGY, v. 11, FEB 14 2020. (14/08458-9, 17/50222-0, 17/50334-3, 16/01960-6)

LEASTRO, MIKHAIL OLIVEIRA; ORTEGA CASTRO, DEIBIS YORLENIS; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS ANGEL. Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. FRONTIERS IN MICROBIOLOGY, v. 11, JUN 9 2020. (15/10249-1, 17/50222-0, 17/19898-8)

FREITAS-ASTUA, JULIANA; RAMOS-GONZALEZ, PEDRO LUIS; ARENA, GABRIELLA DIAS; TASSI, ALINE DANIELE; KITAJIMA, ELLIOT WATANABE. Brevipalpus-transmitted viruses: parallelism beyond a common vector or convergent evolution of distantly related pathogens?. CURRENT OPINION IN VIROLOGY, v. 33, p. 66-73, DEC 2018. (17/50222-0, 14/08458-9)

AMARASINGHE, GAYA K.; AYLLON, MARIA A.; BAO, YIMING; BASLER, CHRISTOPHER F.; BAVARI, SINA; BLASDELL, KIM R.; BRIESE, THOMAS; BROWN, PAUL A.; BUKREYEV, ALEXANDER; BALKEMA-BUSCHMANN, ANNE; et al. Taxonomy of the order Mononegavirales: update 2019. ARCHIVES OF VIROLOGY, v. 164, n. 7, p. 1967-1980, JUL 2019. (16/01960-6, 17/50222-0, 14/08458-9)

ARENA, GABRIELLA D.; RAMOS-GONZALEZ, PEDRO L.; TASSI, ALINE D.; MACHADO, MARCOS A.; FREITAS-ASTUA, JULIANA. A TaqMan RT-qPCR assay for absolute quantification of citrus leprosis virus C lineage SJP: disclosing the subgenomic/genomic ratio in plant and mite vector, plant organ-specific viral loads, and the kinetics of viral accumulation in plants. ROPICAL PLANT PATHOLOG, v. 48, n. 1, p. 12-pg., 2022-11-07. (18/12252-8, 19/02137-0, 17/50222-0)

RAMOS-GONZALEZ, PEDRO LUIS; ARENA, GABRIELLA DIAS; TASSI, ALINE DANIELE; CHABI-JESUS, CAMILA; KITAJIMA, ELLIOT WATANABE; FREITAS-ASTUA, JULIANA. Kitaviruses: A Window to Atypical Plant Viruses Causing Nonsystemic Diseases. ANNUAL REVIEW OF PHYTOPATHOLOGY, v. 61, p. 22-pg., 2023-01-01. (17/50222-0, 20/15413-2, 19/02137-0, 18/12252-8, 17/50334-3, 19/25078-9)

CHABI-JESUS, CAMILA; RAMOS-GONZALEZ, PEDRO L.; POSTCLAM-BARRO, MATHEUS; FONTENELE, RAFAELA SALGADO; HARAKAVA, RICARDO; BASSANEZI, RENATO B.; MOREIRA, ALECIO S.; KITAJIMA, ELLIOT W.; VARSANI, ARVIND; FREITAS-ASTUA, JULIANA. Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas. FRONTIERS IN MICROBIOLOGY, v. 12, APR 29 2021. (16/01960-6, 17/50334-3, 17/50222-0, 19/25078-9, 20/06378-9, 19/08186-2)

RAMOS-GONZALEZ, PEDRO LUIS; DOS SANTOS, GUSTAVO FRANCISCO; CHABI-JESUS, CAMILA; HARAKAVA, RICARDO; KITAJIMA, ELLIOT W.; FREITAS-ASTUA, JULIANA. Passion Fruit Green Spot Virus Genome Harbors a New Orphan ORF and Highlights the Flexibility of the 5 '-End of the RNA2 Segment Across Cileviruses. FRONTIERS IN MICROBIOLOGY, v. 11, p. 16-pg., 2020-02-14. (17/50334-3, 14/08458-9, 16/01960-6, 17/50222-0)

DE LILLO, ENRICO; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; RAMOS-GONZALEZ, PEDRO LUIS; SIMONI, SAURO; TASSI, ALINE DANIELE; VALENZANO, DOMENICO. Phytophagous mites transmitting plant viruses: update and perspectives. ENTOMOLOGIA GENERALIS, v. 41, n. 5, p. 439-462, 2021. (14/08458-9, 17/50222-0, 18/12252-8, 19/25078-9)

TASSI, ALINE DANIELE; RAMOS-GONZALEZ, PEDRO LUIS; SINICO, THAIS ELISE; KITAJIMA, ELLIOT WATANABE; FREITAS-ASTUA, JULIANA. Circulative Transmission of Cileviruses in Brevipalpus Mites May Involve the Paracellular Movement of Virions. FRONTIERS IN MICROBIOLOGY, v. 13, p. 11-pg., 2022-04-06. (17/50334-3, 19/25078-9, 18/12252-8, 17/50222-0)