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Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins

Grant number: 18/18257-1
Support type:Multi-user Equipment Program
Duration: November 01, 2018 - October 31, 2025
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Giuseppe Palmisano
Grantee:Giuseppe Palmisano
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications, AP.JP
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page:
Tipo de equipamento:Caracterização de Materiais - Análises Químicas - Cromatrografia líquida
Fabricante: Thermo Scientific
Modelo: ICS + detectores UV + fluorescência + eletroquímico


We aim at building an analytical platform for glycan identification and quantification. The analysis of mono, oligo and polysaccharides will offer the possibility of studying the modulation of the cellular, tissue and biofluid glycome in different biological systems. Differential glycan expression has been associated to several diseases from cancer, infectious diseases and others. In particular, glycoproteins, glycolipids, proteoglycans, and other glycoconjugates have been suggested as possible candidate biomarkers and therapeutic targets. Due to that, their analysis and structural characterization is important to fully elucidate the glycome in pathophysiological conditions. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, GUILHERME MEIRA; EFFER, BRIAN; BIASOTO, HENRIQUE PELLIN; FEIJOLI, VERONICA; PESSOA, ADALBERTO; PALMISANO, GIUSEPPE; MONTEIRO, GISELE. Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis. Biochemical Engineering Journal, v. 156, APR 15 2020. Web of Science Citations: 0.
KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, SI NOV 2019. Web of Science Citations: 0.
CAPELLI-PEIXOTO, JANAINA; MULE, SIMON NGAO; TANO, FABIA TOMIE; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteomics and Leishmaniasis: Potential Clinical Applications. PROTEOMICS CLINICAL APPLICATIONS, v. 13, n. 6 AUG 2019. Web of Science Citations: 0.

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