Byproducts recovery from food processing: extraction of phytochemical compounds, encapsulation and application in biodegradable films / coatings

• Grant number: 19/23171-1
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2020
• End date: October 31, 2022
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Milena Martelli Tosi
• Grantee: Milena Martelli Tosi
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The aim of this study are (i) to produce extracts of active compounds from byproducts obtained after the processing of acerola puree (Malpighia emarginata) and carrot juice (Daucus carota) using sonication and ethanolic or chitosan solution; (ii) to encapsulate these compounds in chitosan-recovered liposomes by the ionic gelation system using sodium tripolyphosphate (TPP-chitosomes); (iii) to obtain nanofibers from the extraction residue, (iv) to incorporate the particles in biodegradable film/coating and (v) to apply as coating of fruits/vegetables. Initially, an experimental design 22 will be carried out in order to optimize the extraction of phenolic compounds and carotenoids, depending on the byproduct. Byproduct concentration and power output/time of sonication as independent variables. The quantification of phenolic compounds will be performed by spectrophotometry and their identification, at the optimized condition, will be evaluated by Ultra-Efficient Liquid Chromatography - Mass Spectrometry (UPLC-MS). The quantification of carotenoids will be performed with the aid of High Performance Liquid Chromatography (HPLC). TPP-chitosomes will be evaluated according to particle size by dinamic light scattering (DLS) analysis and zeta potential, ATR/FTIR (Attenuated Infrared Spectroscopy/Fourier Transform Infrared Spectroscopy), morphology by Confocal Microscopy or Field Emission Scanning Electron Microscopy and in vtiro digestibility. Coatings/ films will be characterized in relation to their mechanical and functional properties. The release of phytochemical will also be evaluated. These products will be applied to minimally processed food by spraying technique, being analyzed and compared according to loss of mass, firmness, pH, color, and microbiological analysis. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, NATALIA CRISTINA; ASSIS, ODILIO BENEDITO GARRIDO; SARTORI, ALAN GIOVANINI DE OLIVEIRA; DE ALENCAR, SEVERINO MATIAS; MARTELLI-TOSI, MILENA. Chitosan suspension as extractor and encapsulating agent of phenolics from acerola by-product. Food Research International, v. 161, p. 8-pg., 2022-09-05. (16/18788-1, 19/23171-1)

ESPOSTO, BRUNO STEFANI; JAUREGI, PAULA; TAPIA-BLACIDO, DELIA R.; MARTELLI-TOSI, MILENA. Liposomes vs. chitosomes: Encapsulating food bioactives. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 108, p. 40-48, FEB 2021. (19/22502-4, 19/23171-1, 19/05511-0, 16/18788-1)

ESPOSTO, BRUNO STEFANI; TESSARO, LARISSA; AGUILAR, GUILHERME JOSE; SOBRAL, PAULO JOSE DO AMARAL; TAPIA-BLACIDO, DELIA RITA; MARTELLI-TOSI, MILENA. Chitosan films activated with phytochemicals extracted from carrot by-product encapsulated in TPP chitosomes. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 3, p. 10-pg., 2023-12-26. (19/23171-1, 16/18788-1, 19/22502-4, 19/05511-0)

SILVA, NATALIA CRISTINA; SILVA, MAYCON JHONY; ASSIS, ODILIO BENEDITO GARRIDO; MARTELLI-TOSI, MILENA. Ultrasound-assisted extraction of bioactives as a strategic step for chemical pretreatments in nanocellulose production from acerola by-products. International Journal of Biological Macromolecules, v. 276, p. 8-pg., 2024-07-18. (19/23171-1, 22/03055-0)

BASSAN, LUANA TORTELLI; NASCIMENTO, KAREN REBOUCAS; IQUIAPAZA, IVONE YANIRA CHOQUETICO; FERREIRA, MARCIA ELIANA DA SILVA; TAPIA-BLACIDO, DELIA RITA; FABI, JOAO PAULO; MARTELLI-TOSI, MILENA. Chitosan suspension enriched with phenolics extracted from pineapple by-products as bioactive coating for liposomes: Physicochemical properties and in vitro cytotoxicity. Food Research International, v. 201, p. 10-pg., 2025-01-06. (19/23171-1, 13/07914-8)

ESPOSTO, BRUNO STEFANI; BOTELHO PINHO, SABRINA GUARDA; THOMAZINI, MARCELO; RAMOS, ANA PAULA; TAPIA-BLACIDO, DELIA RITA; MARTELLI-TOSI, MILENA. TPP-chitosomes as potential encapsulation system to protect carotenoid-rich extract obtained from carrot by-product: A comparison with liposomes and chitosomes. Food Chemistry, v. 397, p. 8-pg., 2022-08-06. (19/22502-4, 19/05511-0, 16/18788-1, 19/23171-1)

DA SILVA, NATALIA CRISTINA; DE BARROS-ALEXANDRINO, TAIS TEO; GARRIDO ASSIS, ODILIO BENEDITO; MARTELLI-TOSI, MILENA. Extraction of phenolic compounds from acerola by-products using chitosan solution, encapsulation and application in extending the shelf-life of guava. Food Chemistry, v. 354, AUG 30 2021. (16/18788-1, 19/23171-1)

NATALIA CRISTINA DA SILVA; PEDRO LUCAS APOLINARIO DOURADO; MILENA MARTELLI TOSI. A review about acerola (Malpighia emarginata DC.) by-products as a promising raw material for the generation of green products. Brazilian Journal of Food Technology, v. 26, 2023-12-01. (19/23171-1)

SILVA, MARLUCI PALAZZOLLI; MARTELLI-TOSI, MILENA; MASSARIOLI, ADNA PRADO; MELO, PRISCILLA SIQUEIRA; ALENCAR, SEVERINO MATIAS; FAVARO-TRINDADE, CARMEN S.. Co-encapsulation of guaran acute accent a extracts and probiotics increases probiotic survivability and simultaneously delivers bioactive compounds in simulated gastrointestinal fluids. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 161, p. 11-pg., 2022-03-18. (19/09757-3, 16/24895-5, 19/23171-1)