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Collection and analysis of extracts from seeds of pitanga (Eugenia uniflora L.) obtained by use of supercritical carbon dioxide

Grant number: 08/00148-0
Support type:Regular Research Grants
Duration: October 01, 2008 - September 30, 2011
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Alessandra Lopes de Oliveira
Grantee:Alessandra Lopes de Oliveira
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil


The Brazilian industry of Pitanga juice and pulp processing produces 100 ton/year of seeds. Considering the significant amount of waste, the search for natural compounds with phytotherapeutic properties and the preliminary studies conducted in this laboratory, this research project aims to obtain extracts from seeds of Pitanga by use of supercritical CO2 and characterize its composition in order to identify the presence of these compounds. Pitanga seeds have low protein content (4.33%), therefore could be used as a source of protein in animal feed, for example. However, its extract (0.5%) presents refraction index characteristic of essential oils, from 1507 to 1515. Preliminary analysis of gas chromatography and mass spectrometry showed twenty four components (22.36% by mass of terpene) which are also present in the leaves and fruit. A selina-1,3,7-(11)-trien-8-one, component in high concentration in the leaves extracts and, recently synthesized, was also identified in this extract. Thus, this ether extract seeds of Pitanga is rich in components similar to those presented in leaves and fruit, and may also perform phytotherapeutic activities. So, the Pitanga seeds extract is characterized as an oil rich in essential components similar to the leaves and fruit, which can play therapeutic activities. Thus, the seeds, that were treated as waste, can be to generate 500 kg of extract annually with pharmacological and/or cosmetics purposes. In front of these preliminary tests, which are best discused during this presented project,the objective of this work is to obtain extracts of Pitanga seeds using supercritical CO2, studying the efficiency of the extraction at different conditions of pressure and temperature, evaluating its composition and its application for medical, food and cosmetics industries, since the extracts, from this form of extraction, are highly purified and free of organic solvents. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VILLEGAS, MARIA EUGENIA; AREDO, VICTOR; EGG ASEVEDO, KAYQUE JULIO; LOURENCO, RODRIGO VINICIUS; BAZITO, REINALDO CAMINO; OLIVEIRA, ALESSANDRA LOPES. Commercial Starch Behavior When Impregnated with Food Additives by Moderate Temperature Supercritical CO(2)Processing. STARCH-STÄRKE, APR 2020. Web of Science Citations: 0.
MACHADO, L. C.; PELEGATI, V. B.; OLIVEIRA, A. L. Study of simple microparticles formation of limonene in modified starch using PGSS - Particles from gas-saturated suspensions. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 260-269, JAN 2016. Web of Science Citations: 3.
SANTOS, DEBORA NASCIMENTOE; DE SOUZA, LARISSA LIMA; FERNANDES DE OLIVEIRA, CARLOS AUGUSTO; SILVA, EDSON ROBERTO; DE OLIVEIRA, ALESSANDRA LOPES. Arginase inhibition, antibacterial and antioxidant activities of Pitanga seed (Eugenia uniflora L.) extracts from sustainable technologies of high pressure extraction. FOOD BIOSCIENCE, v. 12, p. 93-99, DEC 1 2015. Web of Science Citations: 4.
NASCIMENTO E SANTOS, DEBORA; DE SOUZA, LARISSA LIMA; FERREIRA, NILSON JOSE; DE OLIVEIRA, ALESSANDRA LOPES. Study of supercritical extraction from Brazilian cherry seeds (Eugenia uniflora L.) with bioactive compounds. FOOD AND BIOPRODUCTS PROCESSING, v. 94, p. 365-374, APR 2015. Web of Science Citations: 6.
OLIVEIRA, ALESSANDRA L.; DESTANDAU, EMILIE; FOUGERE, LAETITIA; LAFOSSE, MICHEL. Isolation by pressurised fluid extraction (PFE) and identification using CPC and HPLC/ESI/MS of phenolic compounds from Brazilian cherry seeds (Eugenia uniflora L.). Food Chemistry, v. 145, p. 522-529, FEB 15 2014. Web of Science Citations: 23.
MALAMAN, FELIPE S.; MORAES, LUIZ ALBERTO B.; WEST, CAROLINE; FERREIRA, NILSON J.; OLIVEIRA, ALESSANDRA L. Supercritical fluid extracts from the Brazilian cherry (Eugenia uniflora L.): Relationship between the extracted compounds and the characteristic flavour intensity of the fruit. Food Chemistry, v. 124, n. 1, p. 85-92, JAN 1 2011. Web of Science Citations: 23.

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