Fluorescence system for investigations of physiological and pathophysiological aspects in cellular models

• Grant number: 09/53840-0
• Support Opportunities: Multi-user Equipment Program
• Start date: September 01, 2010
• End date: December 31, 2012
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Adriana Karaoglanovic Carmona
• Grantee: Adriana Karaoglanovic Carmona
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

In this project, we intend to enable pathophysiology studies in experimental models that are being developed by the researchers involved. The topics that will be explored by the researchers are of great medical and biological interest and could answer important questions related to pathologies of high incidence in humans. Among other topics, the projects presented are related to the following: arterial hypertension and its involvement in heart disease (participation of the renin-angiotensin and kallikrein-kinin systems); liver metabolism in the setting of inflammation; the effects of sleep deprivation on physiology and cellular responses; deficiency and activation of various membrane receptors; apoptosis (cell death); study of intracellular proteases; and the development of new fluorescent probes. These projects could allow us to develop and apply new methodologies for study of the intracellular metabolism in various experimental models, identifying associations with the mechanisms of signaling, ionic mobility, and morphology of organelles, as well as with the expression and activity of proteins, or rather, making it possible to understand the complexity of the cell physiology involved in certain pathologies. Therefore, the fluorescence system requested is essential for the analysis (distribution/ morphometry and quantification) of fluorescence in fixed preparations or in cell cultures. Accessories such as an environmentally controlled chamber (with regulation of temperature, CO2, and humidity) and slide holders could be employed in the study of live cells, making it possible to create a controlled environment for metabolic, pharmacological and studies of cell signaling in multiple culture plates. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMARGO, ROSALINDA YASATO; KANAMURA, CRISTINA TAKAMI; FRIGUGLIETTI, CELSO UBIRAJARA; NOGUEIRA, CELIA REGINA; IORCANSKY, SONIA; TINCANI, ALFIO JOSE; BEZERRA, ANA KARINA; BRUST, ESTER; KOYAMA, FERNANDA CHRISTTANINI; CAMARGO, ANAMARIA ARANHA; et al. Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, 2018. (14/24549-4, 10/12005-9, 09/53840-0)

LAMARTINE-HANEMANN, SIMONE DA SILVA; FERREIRA-SILVA, GUILHERME ALVARO; HORVATH, RENATO DE OLIVEIRA; SONCINI, ROSELI; CAIXETA, ESTER SIQUEIRA; ROCHA-SALES, BIANCA; NIERO, EVANDRO LUIS; MACHADO-SANTELLI, GLAUCIA MARIA; DOS SANTOS, MARCELO HENRIQUE; DE OLIVEIRA, JAQUELINE CARVALHO; et al. A tetraprenylated benzophenone 7-epiclusianone induces cell cycle arrest at G1/S transition by modulating critical regulators of cell cycle in breast cancer cell lines. TOXICOLOGY IN VITRO, v. 68, OCT 2020. (09/53840-0)

MELO, POLLYANA M. S.; MALUF, SARAH EL CHAMY; AZEVEDO, MAURO F.; PASCHOALIN, THAYSA; BUDU, ALEXANDRE; BAGNARESI, PIERO; HENRIQUE-SILVA, FLAVIO; SOARES-COSTA, ANDREA; GAZARINI, MARCOS L.; CARMONA, ADRIANA K.. Inhibition of Plasmodium falciparum cysteine proteases by the sugarcane cystatin CaneCPI-4. Parasitology International, v. 67, n. 2, p. 233-236, APR 2018. (15/06861-3, 15/19316-3, 16/15298-3, 11/14403-4, 13/12913-0, 09/53840-0)

BRITO DA SILVA, ANNIELLE MENDES; SILVA-GONCALVES, LAIZ COSTA; OLIVEIRA, FERNANDO AUGUSTO; ARCISIO-MIRANDA, MANOEL. Pro-necrotic Activity of Cationic Mastoparan Peptides in Human Glioblastoma Multiforme Cells Via Membranolytic Action. Molecular Neurobiology, v. 55, n. 7, p. 5490-5504, JUL 2018. (12/02065-0, 16/13368-4, 12/50336-2, 09/53840-0)

DE SOUZA, I; MONTEIRO, L. K. S.; GUEDES, C. B.; SILVA, M. M.; ANDRADE-TOMAZ, M.; CONTIERI, B.; LATANCIA, M. T.; MENDES, D.; PORCHIA, B. F. M. M.; LAZARINI, M.; et al. High levels of NRF2 sensitize temozolomide-resistant glioblastoma cells to ferroptosis via ABCC1/MRP1 upregulation. CELL DEATH & DISEASE, v. 13, n. 7, p. 13-pg., 2022-07-08. (19/19435-3, 19/26268-6, 19/15320-7, 13/07467-1, 19/21745-0, 19/27080-0, 09/53840-0)

PASCHOALIN, THAYSA; MARTENS, ADAM A.; OMORI, ALVARO T.; PEREIRA, FELIPE V.; JULIANO, LUIZ; TRAVASSOS, LUIZ R.; MACHADO-SANTELLI, GLAUCIA M.; CUNHA, RODRIGO L. O. R.. Antitumor effect of chiral organotelluranes elicited in a murine melanoma model. Bioorganic & Medicinal Chemistry, v. 27, n. 12, SI, p. 2537-2545, JUN 15 2019. (09/53840-0)

GOMES, MAYRIM M.; BUDU, ALEXANDRE; VENTURA, PRISCILLA D. S.; BAGNARESI, PIERO; COTRIN, SIMONE S.; CUNHA, RODRIGO L. O. R.; CARMONA, ADRIANA K.; JULIANO, LUIZ; GAZARINI, MARCOS L.. Specific calpain activity evaluation in Plasmodium parasites. Analytical Biochemistry, v. 468, p. 22-27, JAN 1 2015. (10/09264-2, 11/15287-8, 13/12913-0, 09/53840-0)

MELO, POLLYANA M. S.; BAGNARESI, PIERO; PASCHOALIN, THAYSA; HIRATA, IZAURA Y.; GAZARINI, MARCOS L.; CARMONA, ADRIANA K.. Plasmodium falciparum proteases hydrolyze plasminogen, generating angiostatin-like fragments. Molecular and Biochemical Parasitology, v. 193, n. 1, p. 45-54, JAN 2014. (10/51423-0, 09/11261-4, 09/53840-0)

CAMARGO, ROSALINDA YASATO; KANAMURA, CRISTINA TAKAMI; FRIGUGLIETTI, CELSO UBIRAJARA; NOGUEIRA, CELIA REGINA; IORCANSKY, SONIA; TINCANI, ALFIO JOSE; BEZERRA, ANA KARINA; BRUST, ESTER; KOYAMA, FERNANDA CHRISTTANINI; CAMARGO, ANAMARIA ARANHA; et al. Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, v. 2018, p. 10-pg., 2018-01-01. (14/24549-4, 10/12005-9, 09/53840-0)

PASCHOALIN, THAYSA; MARTENS, ADAM A.; OMORI, ALVARO T.; PEREIRA, FELIPE V.; JULIANO, LUIZ; TRAVASSOS, LUIZ R.; MACHADO-SANTELLI, GLAUCIA M.; CUNHA, RODRIGO L. O. R.. Antitumor effect of chiral organotelluranes elicited in a murine melanoma model. Bioorganic & Medicinal Chemistry, v. 27, n. 12, p. 9-pg., 2019-06-15. (09/53840-0)