Interaction of bioactive materials in ultrathin films organized in models for biointerfaces models for investigation of molecular recognition processes and associated molecular mechanisms

• Grant number: 13/10213-1
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2013
• End date: August 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Luciano Caseli
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

In this project, we intend to immobilize bioactive materials, natural or synthetic, in interfaces considered as bioinspired, organized at the air-water interface (Gibbs and Langmuir films) or at the solid-air interface (Langmuir-Blodgett films). Materials with pharmacological and medical interest are candidates. They are those that mainly interfere in events that occur in molecules associated to cell membranes. Of particular interest are materials acting on cell signaling that involves transmembrane receptors, characterizing their expression in tumor cells, or those materials that acts simply by destroying the cell membrane of parasites, being considered therefore as antimicrobial or antifungal. In addition, we intend to immobilize enzymes in membrane models, in order to take advantage of its ability related to molecular recognition and the ability to incorporate it in a system considered bioinspired. As a result this project intends to strengthen the already-existing collaboration between the proponent researcher with other institution's groups, which are particularly expert either in obtaining bioactive materials, or in Molecular Biology of transmembrane receptors. This project therefore aims to connect these areas with the investigation of membrane models organized at the air-water interface. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES, GIULIA E. G.; DE SOUZA, FERNANDA S.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. The interaction of eugenol with cell membrane models at the air-water interface is modulated by the lipid monolayer composition. Biophysical Chemistry, v. 207, p. 7-12, DEC 2015. (13/10213-1)

SOARES, CECILIA GONCALVES; CASELI, LUCIANO; BERTUZZI, DIEGO LUAN; SANTOS, FABIO SANTANA; GARCIA, JAREM RAUL; PERES, LAURA OLIVEIRA. Ultrathin films of poly(2,5-dicyano-p-phenylene-vinylene)-co-(p-phenylene-vinylene) DCN-PPV/PPV: A Langmuir and Langmuir-Blodgett films study. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 467, p. 201-206, FEB 20 2015. (13/10213-1)

GOTO, THIAGO EICHI; CASELI, LUCIANO. The interaction of mefloquine hydrochloride with cell membrane models at the air-water interface is modulated by the monolayer lipid composition. Journal of Colloid and Interface Science, v. 431, p. 24-30, OCT 1 2014. (13/10213-1)

NARDUCCI FERRREIRA, JOAO VICTOR; GRECCO, SIMONE DOS S.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Ultrathin films of lipids to investigate the action of a flavonoid with cell membrane models. Materials Science & Engineering C-Materials for Biological Applications, v. 48, p. 112-117, MAR 1 2015. (13/10213-1)

SAKAI, ANDREI; PERES, LAURA O.; CASELI, LUCIANO. Langmuir and Langmuir-Blodgett films of Cl-PPV mixed with stearic acid: implication of the morphology on the surface and spectroscopy properties. COLLOID AND POLYMER SCIENCE, v. 293, n. 3, p. 883-890, MAR 2015. (13/10213-1)

DE ARAUJO, FELIPE TEJADA; CASELI, LUCIANO. Rhodanese incorporated in Langmuir and Langmuir-Blodgett films of dimyristoylphosphatidic acid: Physical chemical properties and improvement of the enzyme activity. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 141, p. 59-64, MAY 1 2016. (13/10213-1)

DE BRITO, AUDREY KALINOUSKI; NORDI, CRISTINA S. F.; CASELI, LUCIANO. Algal polysaccharides as matrices for the immobilization of urease in lipid ultrathin films studied with tensiometry and vibrational spectroscopy: Physical-chemical properties and implications in the enzyme activity. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 135, p. 639-645, NOV 1 2015. (11/14289-7, 13/10213-1)

FERREIRA, JOAO VICTOR N.; CAPELLO, TABATA M.; SIQUEIRA, LEONARDO J. A.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Mechanism of Action of Thymol on Cell Membranes Investigated through Lipid Langmuir Monolayers at the Air-Water Interface and Molecular Simulation. Langmuir, v. 32, n. 13, p. 3234-3241, APR 5 2016. (15/23446-0, 13/10213-1)

ROCHA, JEFFERSON MUNIZ; CASELI, LUCIANO. Adsorption and enzyme activity of sucrose phosphorylase on lipid Langmuir and Langmuir-Blodgett films. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 116, p. 497-501, APR 1 2014. (13/10213-1)

SCHOLL, FABIO ANTONIO; CASELI, LUCIANO. Langmuir and Langmuir-Blodgett films of lipids and penicillinase: Studies on adsorption and enzymatic activity. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 126, p. 232-236, FEB 1 2015. (13/10213-1)