| Grant number: | 13/05083-1 |
| Support Opportunities: | Regular Research Grants |
| Start date: | September 01, 2013 |
| End date: | November 30, 2015 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Reproduction |
| Principal Investigator: | Marcelo Fábio Gouveia Nogueira |
| Grantee: | Marcelo Fábio Gouveia Nogueira |
| Host Institution: | Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil |
| City of the host institution: | Assis |
| Associated researchers: | Ciro Moraes Barros ; Eduardo Montanari Razza ; Mariana Fernandes Machado |
Abstract
In vitro maturation (IVM) restricts the use of hormones in vivo preventing excessive spending and handling of the animal, and the harmful iatrogenic effects of ovarian overstimulation. However, the IVM systems are not comparably efficient to physiological events that occur in vivo, mainly concerning the synchronization between the nuclear and cytoplasmic maturation of the oocyte. Removed from the follicular environment (in vitro), break up the gap junctions, resulting in low levels of cAMP in the oocyte allowing resumption of meiosis and consequent spontaneous asynchronous cytoplasmic maturation. Simulated physiological oocyte maturation (SPOM) provides a complex pharmacological manipulation of IVM. This system inhibits meiotic progression in two distinct phases (pre-maturation and maturation), while it stimulates oocyte maturation with high doses of FSH, achieving better results compared to conventional IVM. Another bias of investigation regard meiotic arrest comprises natriuretic peptide precursor type C (NPPC) and its receptor (NPR2) system. The interaction between these molecules in cumulus cells, indirectly contributes to the maintenance of cAMP levels, leading to meiotic arrest. Therefore, the study aims to assess the overall panel of bovine oocyte maturation in vitro. Specifically, the project aim to elucidate NPPC-NPR2 meiotic arrest system and its potential application in IVM routine; to compare SPOM and conventional IVM overall and in the oocytes and cumulus cells transcriptomics and embryo produced. Finally, the project will provide robust data for the development of systems that maximize in vitro production of bovine embryos and that will be substantially availed by the thematic project (GIFT) recently granted by FAPESP (2012/50533-2). (AU)
| Articles published in Agência FAPESP Newsletter about the research grant: |
| More itemsLess items |
| TITULO |
| Articles published in other media outlets ( ): |
| More itemsLess items |
| VEICULO: TITULO (DATA) |
| VEICULO: TITULO (DATA) |