| Grant number: | 14/07837-6 |
| Support Opportunities: | Regular Research Grants |
| Start date: | June 01, 2014 |
| End date: | May 31, 2016 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Pathology |
| Principal Investigator: | Terezinha Knöbl |
| Grantee: | Terezinha Knöbl |
| Host Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| City of the host institution: | São Paulo |
Abstract
Some species of Arcobacter are associated with food poisoning and may represent a biological hazard, associated frequently with poultry or porcine meat consume. In Brazil, this agent has been poor studied and there are few epidemiological studies in Southern region. The aim of this project is to characterize The antimicrobial profile of Arcobacter species, isolated from parts of chickens and porcine meat marketed in São Paulo, as well as perform a phylogenetic analysis of these isolates. A total of 200 cuts of breast, wing, thigh and drumstick of chicken and 200 cuts of prime rib, sirloin, steak and pork shank, coming from local markets, butchers and small markets in São Paulo will be assessed. After the step of pre-enrichment, samples are grown in a selective medium, Johnson and Murano. Bacterial species will be identified by molecular techniques (Polymerase chain reaction - PCR). The antimicrobial susceptibility profile will be determined by the minimum inhibitory concentration (MIC), Genotypic profile of the isolates will be evaluated by the AFLP technique (length polymorphism of amplified fragments). (AU)
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