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Serotonin and respiratory control in vertebrates

Grant number: 13/17606-9
Support type:Research Grants - Young Investigators Grants
Duration: June 01, 2014 - December 31, 2017
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Glauber dos Santos Ferreira da Silva
Grantee:Glauber dos Santos Ferreira da Silva
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Assoc. researchers:Luciane Helena Gargaglioni Batalhão ; Luiz Guilherme de Siqueira Branco
Associated scholarship(s):17/10868-9 - Medullary raphe and active expiration during hypercapnia: role of serotonergic modulation, BP.IC
16/11061-9 - Active expiration and sleep-wake cycle: participation of medullary raphe, BP.IC
15/24785-2 - The role of TRPV1 channels in the cardiorespiratory responses to hypercapnia and hypoxia in unanaesthetized Wistar rats, BP.IC
15/04926-0 - Bulbar raphe role in active expiration modulation during hypercapnia in different arousal states (sleep and wakefulness), BP.IC
14/12190-1 - Serotonin and respiratory control in vertebrates, BP.JP

Abstract

The broad distribution of serotonin (5-HT) in various organisms indicates that this is a phylogenetically ancient system. In the nervous system, 5-HT is involved in several functions, including circadian rhythms, diet, emotional state, control of sleep and respiratory control. In mammals, respiration is a rhythmic process, initiated in the central nervous system (CNS). At rest, expiration is a passive process, however, mammals recruit expiratory muscles (such as abdominal) under conditions of increased respiratory drive, such as during hypercapnia. Studying active expiration is critical, as it constitutes an important defense mechanism, activated under conditions essential to generate forced expiration. It also may be involved in pathological conditions. Studies have suggested that the RTN/pFRG is the main generator site of this activity. There are few studies on motor control and respiratory-related activity of the abdominal muscles (expiration), and there is no description of any other brainstem regions that may modulate this activity. In particular, medullary raphe 5-HT neurons, which are involved in the central CO2/pH chemoreception, since hypercapnia is a condition in which active expiration is present. The medullary raphe 5-HT neurons are considered to be an important component of CO2/pH chemoreception and through excitatory projections to RTN may influence and modulate active expiration. This type of serotonergic modulation on active expiration during hypercapnia has never been demonstrated, particularly in non-anesthetized animals and during sleep and wakefulness. In addition, the project will also address comparative aspects of 5-HT and respiratory control in vertebrates. In mammals, the role and involvement of 5-HT neurons in central chemoreception is well known. While this topic has been focused in mammals, few studies have been conducted in other vertebrate classes. Therefore, this project also aims to study the role of 5-HT neurons in the ventilatory response to hypercapnia, using two animal models: the lungfish (Lepidosiren paradoxa, known as Pirambóia) and birds (chicken, Gallus gallus). The evaluation of the involvement of 5-HT system in central chemoreception in these two classes of animals is unprecedented in the literature. All experiments will be performed in non-anesthetized animals and 5-HT receptors agonist and antagonist as well as specific chemical lesion of 5-HT neurons will be applied to test the involvement of 5-HT system on the respiratory variables proposed in the study presently. (AU)

Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NUNAN, BRUNA L. C. Z.; SILVA, AYLA S.; WANG, TOBIAS; DA SILVA, GLAUBER S. F. Respiratory control of acid-base status in lungfish. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 237, NOV 2019. Web of Science Citations: 0.
MINTO, WALTER J.; GIUSTI, HUMBERTO; GLASS, MOGENS L.; KLEIN, WILFRIED; DA SILVA, GLAUBER S. F. Buccal jet streaming and dead space determination in the South American lungfish, Lepidosiren paradoxa. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 235, p. 159-165, SEP 2019. Web of Science Citations: 0.
LEIRAO, ISABELA P.; SILVA, CARLOS A.; GARGAGLIONI, LUCIANE H.; DA SILVA, GLAUBER S. F. Hypercapnia-induced active expiration increases in sleep and enhances ventilation in unanaesthetized rats. JOURNAL OF PHYSIOLOGY-LONDON, v. 596, n. 15, p. 3271-3283, AUG 1 2018. Web of Science Citations: 4.
ZENA, LUCAS A.; LEITE, CLEO A. C.; LONGHINI, LEONARDO S.; DIAS, DANIEL P. M.; DA SILVA, GLAUBER S. F.; HARTZLER, LYNN K.; GARGAGLIONI, LUCIANE H.; BICEGO, KENIA C. Analysis of the respiratory component of heart rate variability in the Cururu toad Rhinella schneideri. SCIENTIFIC REPORTS, v. 7, NOV 23 2017. Web of Science Citations: 3.
MARQUES, DANUZIA A.; DE CARVALHO, DEBORA; DA SILVA, GLAUBER S. F.; SZAWKA, RAPHAEL E.; ANSELMO-FRANCI, JANETE A.; BICEGO, KENIA C.; GARGAGLIONI, LUCIANE H. Influence of estrous cycle hormonal fluctuations and gonadal hormones on the ventilatory response to hypoxia in female rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, v. 469, n. 10, p. 1277-1286, OCT 2017. Web of Science Citations: 6.
DA SILVA, GLAUBER S. F.; SABINO, JOAO P. J.; RAJANI, VISHAAL; ALVARES, TUCAAUE S.; PAGLIARDINI, SILVIA; BRANCO, LUIZ G. S.; FUNK, GREGORY D. Excitatory Modulation of the preBotzinger Complex Inspiratory Rhythm Generating Network by Endogenous Hydrogen Sulfide. FRONTIERS IN PHYSIOLOGY, v. 8, JUN 30 2017. Web of Science Citations: 1.
DA SILVA, GLAUBER S. F.; VENTURA, DANIELA A. D. N.; ZENA, LUCAS A.; GIUSTI, HUMBERTO; GLASS, MOGENS L.; KLEIN, WILFRIED. Effects of aerial hypoxia and temperature on pulmonary breathing pattern and gas exchange in the South American lungfish, Lepidosiren paradoxa. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 207, p. 107-115, MAY 2017. Web of Science Citations: 3.
ZENA, LUCAS A.; BICEGO, KENIA C.; DA SILVA, GLAUBER S. F.; GIUSTI, HUMBERTO; GLASS, MOGENS L.; SANCHEZ, ADRIANA P. Acute effects of temperature and hypercarbia on cutaneous and branchial gas exchange in the South American lungfish, Lepidosiren paradoxa. Journal of Thermal Biology, v. 63, p. 112-118, JAN 2017. Web of Science Citations: 3.
GRANJEIRO, ERICA MARIA; DA SILVA, GLAUBER S. F.; GIUSTI, HUMBERTO; OLIVEIRA, JOSE ANTONIO; GLASS, MOGENS LESNER; GARCIA-CAIRASCO, NORBERTO. Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain. PLoS One, v. 11, n. 5 MAY 5 2016. Web of Science Citations: 5.
MARQUES, DANUZIA A.; DE CARVALHO, DEBORA; DA SILVA, GLAUBER S. F.; SZAWKA, RAPHAEL E.; ANSELMO-FRANCI, JANETE A.; BICEGO, KENIA C.; GARGAGLIONI, LUCIANE H. Ventilatory, metabolic, and thermal responses to hypercapnia in female rats: effects of estrous cycle, ovariectomy, and hormonal replacement. Journal of Applied Physiology, v. 119, n. 1, p. 61-68, JUL 1 2015. Web of Science Citations: 8.

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