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Multi-User Equipment approved in grant 13/08711-3: mass spectrometer waters SYNAPT G2-Si HDMS + nanoACQUITY UPLC

Grant number: 14/10068-4
Support type:Multi-user Equipment Program
Duration: September 01, 2014 - August 31, 2016
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Daniel Martins-de-Souza
Grantee:Daniel Martins-de-Souza
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/08711-3 - Developing a predictive test for a successful medication response and understanding the molecular bases of schizophrenia through proteomics, AP.JP
EMU web page: Página do Equipamento Multiusuário não informada
Use scheduling: E-mail de agendamento não informado

Abstract

A mass spectrometer is the central core of a proteomics laboratory. Proteomic analyses depend 100% on a mass spectrometer. The equipment proposed is pivotal for the success of the project 13/08711-3 funded by FAPESP. All steps proposed by this project as well as collaborations regarding proteomic analyses in all senses depend heavily on this equipment: protein identification, quantification, top-down proteomics, and large-scale studies of post-translation modifications. Moreover, metabolomics is one more area intended to be covered by this equipment in the long run. The proposed system was chosen based in its capacity of measuring samples in a data independent analyses manner. This is particularly important for clinical projects, since samples can be measured individually: more samples can be analyzed increasing the statistical power. Consequently, results go towards clinical applications. Also, the proteomes of interest can be quantified by a label-free approach, avoiding sample manipulation variations. Additionally, the ion mobility function favors the analyses of post-translational modifications while ETD (Electron-transfer dissociation) may favor top-down approaches. These functions broaden the range of collaborations, which is necessary for a multi-user equipment. (AU)

Articles published in Agência FAPESP about the research grant
Study associates schizophrenia with defective processing of messenger RNA in cells 
Study shows how Zika virus kills brain cells 
Estudo revela como o vírus Zika mata as células cerebrais 

Scientific publications (23)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS DE OLIVEIRA, PERCILLIA VICTORIA; GARCIA-ROSA, SHEILA; AZEVEDO SACHETTO, ANA TERESA; SOARES MORETTI, ANA IOCHABEL; DEBBAS, VICTOR; DE BESSA, TIPHANY CORALIE; SILVA, NATHALIA TENGUAN; PEREIRA, ALEXANDRE DA COSTA; MARTINS-DE-SOUZA, DANIEL; SANTORO, MARCELO LARAMI; MARTINS LAURINDO, FRANCISCO RAFAEL. Protein disulfide isomerase plasma levels in healthy humans reveal proteomic signatures involved in contrasting endothelial phenotypes. REDOX BIOLOGY, v. 22, APR 2019. Web of Science Citations: 0.
BRANDAO-TELES, CAROLINE; DE ALMEIDA, VALERIA; CASSOLI, JULIANA S.; MARTINS-DE-SOUZA, DANIEL. Oligodendrocytes: Potential of Discovering New Treatment Targets. FRONTIERS IN PHARMACOLOGY, v. 10, MAR 5 2019. Web of Science Citations: 1.
DE ALMEIDA, VALERIA; MARTINS-DE-SOUZA, DANIEL. Cannabinoids and glial cells: possible mechanism to understand schizophrenia. EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE, v. 268, n. 7, p. 727-737, OCT 2018. Web of Science Citations: 0.
SUSSULINI, ALESSANDRA; ERBOLATO, HELENA MUNHOZ; PESSOA, GUSTAVO DE SOUZA; ZEZZI ARRUDA, MARCO AURELIO; STEINER, JOHANN; MARTINS-DE-SOUZA, DANIEL. Elemental fingerprinting of schizophrenia patient blood plasma before and after treatment with antipsychotics. EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE, v. 268, n. 6, p. 565-570, SEP 2018. Web of Science Citations: 0.
AQUINO, ADRIANO; ALEXANDRINO, GUILHERME L.; GUEST, PAUL C.; AUGUSTO, FABIO; GOMES, ALEXANDRE F.; MURGU, MICHAEL; STEINER, JOHANN; MARTINS-DE-SOUZA, DANIEL. Blood-Based Lipidomics Approach to Evaluate Biomarkers Associated With Response to Olanzapine, Risperidone, and Quetiapine Treatment in Schizophrenia Patients. FRONTIERS IN PSYCHIATRY, v. 9, MAY 25 2018. Web of Science Citations: 2.
VELASQUEZ, ERIKA; NOGUEIRA, FABIO C. S.; VELASQUEZ, INGRID; SCHRNITT, ANDREA; FALKAI, PETER; DOMONT, GILBERTO B.; MARTINS-DE-SOUZA, DANIEL. Synaptosomal Proteome of the Orbitofrontal Cortex from Schizophrenia Patients Using Quantitative Label-Free and iTRAQ-Based Shotgun Proteomics. JOURNAL OF PROTEOME RESEARCH, v. 16, n. 12, p. 4481-4494, DEC 2017. Web of Science Citations: 7.
CASSOLI, JULIANA S.; BRANDAO-TELES, CAROLINE; SANTANA, ALINE G.; SOUZA, GUSTAVO H. M. F.; MARTINS-DE-SOUZA, DANIEL. Ion Mobility-Enhanced Data-Independent Acquisitions Enable a Deep Proteomic Landscape of Oligodendrocytes. PROTEOMICS, v. 17, n. 21 NOV 2017. Web of Science Citations: 3.
DAKIC, VANJA; NASCIMENTO, JULIANA MINARDI; SARTORE, RAFAELA COSTA; MACIEL, RENATA DE MORAES; DE ARAUJO, DRAULIO B.; RIBEIRO, SIDARTA; MARTINS-DE-SOUZA, DANIEL; REHEN, STEVENS K. Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT. SCIENTIFIC REPORTS, v. 7, OCT 9 2017. Web of Science Citations: 8.
ZUCCOLI, GIULIANA S.; SAIA-CEREDA, VERONICA M.; NASCIMENTO, JULIANA M.; MARTINS-DE-SOUZA, DANIEL. The Energy Metabolism Dysfunction in Psychiatric Disorders Postmortem Brains: Focus on Proteomic Evidence. FRONTIERS IN NEUROSCIENCE, v. 11, SEP 7 2017. Web of Science Citations: 6.
TURCK, CHRISTOPH W.; GUEST, PAUL C.; MACCARRONE, GIUSEPPINA; ISING, MARCUS; KLOIBER, STEFAN; LUCAE, SUSANNE; HOLSBOER, FLORIAN; MARTINS-DE-SOUZA, DANIEL. Proteomic Differences in Blood Plasma Associated with Antidepressant Treatment Response. FRONTIERS IN MOLECULAR NEUROSCIENCE, v. 10, AUG 31 2017. Web of Science Citations: 0.
DEZONNE, ROMULO SPERDUTO; SARTORE, RAFAELA COSTA; NASCIMENTO, JULIANA MINARDI; SAIA-CEREDA, VERONICA M.; ROMAO, LUCIANA FERREIRA; ALVES-LEON, SONIZA VIEIRA; DE SOUZA, JORGE MARCONDES; MARTINS-DE-SOUZA, DANIEL; REHEN, STEVENS KASTRUP; ALCANTARA GOMES, FLVIA CARVALHO. Derivation of Functional Human Astrocytes from Cerebral Organoids. SCIENTIFIC REPORTS, v. 7, MAR 27 2017. Web of Science Citations: 12.
GARCEZ, PATRICIA P.; NASCIMENTO, JULIANA MINARDI; DE VASCONCELOS, JANAINA MOTA; DA COSTA, RODRIGO MADEIRO; DELVECCHIO, RODRIGO; TRINDADE, PABLO; LOIOLA, ERICK CORREIA; HIGA, LUIZA M.; CASSOLI, JULIANA S.; VITORIA, GABRIELA; SEQUEIRA, PATRICIA C.; SOCHACKI, JAROSLAW; AGUIAR, RENATO S.; FUZII, HELLEN THAIS; BISPO DE FILIPPIS, ANA M.; GONCALVES VIANEZ JUNIOR, JOAO LIDIO DA SILVA; TANURI, AMILCAR; MARTINS-DE-SOUZA, DANIEL; REHEN, STEVENS K. Zika virus disrupts molecular fingerprinting of human neurospheres. SCIENTIFIC REPORTS, v. 7, JAN 23 2017. Web of Science Citations: 23.
CAFE-MENDES, C. C.; FERRO, E. S.; TORRAO, A. S.; CRUNFLI, F.; RIOLI, V.; SCHMITT, A.; FALKAI, P.; BRITTO, L. R.; TURCK, C. W.; MARTINS-DE-SOUZA, D. Peptidomic analysis of the anterior temporal lobe and corpus callosum from schizophrenia patients. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 97-105, JAN 16 2017. Web of Science Citations: 10.
TAVARES, RAPHAEL; WAJNBERG, GABRIEL; SCHERER, NICOLE DE MIRANDA; PAULETTI, BIANCA ALVES; CASSOLI, JULIANA S.; FERREIRA, CARLOS GIL; PAES LEME, ADRIANA FRANCO; DE ARAUJO-SOUZA, PATRICIA SAVIO; MARTINS-DE-SOUZA, DANIEL; PASSETTI, FABIO. Unveiling alterative splice diversity from human oligodendrocyte proteome data. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 293-301, JAN 16 2017. Web of Science Citations: 1.
SCHMITT, ANDREA; MARTINS-DE-SOUZA, DANIEL; AKBARIAN, SCHAHRAM; CASSOLI, JULIANA S.; EHRENREICH, HANNELORE; FISCHER, ANDRE; FONTEH, ALFRED; GATTAZ, WAGNER F.; GAWLIK, MICHAEL; GERLACH, MANFRED; GRUNBLATT, EDNA; HALENE, TOBIAS; HASAN, ALKOMIET; HASHIMOTO, KENIJ; KIM, YONG-KU; KIRCHNER, SOPHIE-KATHRIN; KORNHUBER, JOHANNES; KRAUS, THEO F. J.; MALCHOW, BEREND; NASCIMENTO, JULIANA M.; ROSSNER, MORITZ; SCHWARZ, MARKUS; STEINER, JOHANN; TALIB, LEDA; THIBAUT, FLORENCE; RIEDERER, PETER; FALKAI, PETER; FORCE, MEMBERS WFSBP TASK. Consensus paper of the WFSBP Task Force on Biological Markers: Criteria for biomarkers and endophenotypes of schizophrenia, part III: Molecular mechanisms. WORLD JOURNAL OF BIOLOGICAL PSYCHIATRY, v. 18, n. 5, p. 330-356, 2017. Web of Science Citations: 4.
SAIA-CEREDA, VERONICA M.; CASSOLI, JULIANA S.; SCHMITT, ANDREA; FALKAI, PETER; MARTINS-DE-SOUZA, DANIEL. Differential proteome and phosphoproteome may impact cell signaling in the corpus callosum of schizophrenia patients. SCHIZOPHRENIA RESEARCH, v. 177, n. 1-3, p. 70-77, NOV 2016. Web of Science Citations: 7.
VENDRAMINI, PEDRO H.; GATTAZ, WAGNER F.; SCHMITT, ANDREA; FALKAI, PETER; EBERLIN, MARCOS N.; MARTINS-DE-SOUZA, DANIEL. Pioneering ambient mass spectrometry imaging in psychiatry: Potential for new insights into schizophrenia. SCHIZOPHRENIA RESEARCH, v. 177, n. 1-3, p. 67-69, NOV 2016. Web of Science Citations: 5.
CASSOLI, JULIANA S.; IWATA, KEIKO; STEINER, JOHANN; GUEST, PAUL C.; TURCK, CHRISTOPH W.; NASCIMENTO, JULIANA M.; MARTINS-DE-SOUZA, DANIEL. Effect of MK-801 and Clozapine on the Proteome of Cultured Human Oligodendrocytes. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 10, MAR 3 2016. Web of Science Citations: 13.
MARTINS-DE-SOUZA, DANIEL; CASSOLI, JULIANA S.; NASCIMENTO, JULIANA M.; HENSLEY, KENNETH; GUEST, PAUL C.; PINZON-VELASCO, ANDRES M.; TURCK, CHRISTOPH W. The protein interactome of collapsin response mediator protein-2 (CRMP2/DPYSL2) reveals novel partner proteins in brain tissue. PROTEOMICS CLINICAL APPLICATIONS, v. 9, n. 9-10, SI, p. 817-831, OCT 2015. Web of Science Citations: 13.
SAIA-CEREDA, VERONICA M.; CASSOLI, JULIANA S.; SCHMITT, ANDREA; FALKAI, PETER; NASCIMENTO, JULIANA M.; MARTINS-DE-SOUZA, DANIEL. Proteomics of the corpus callosum unravel pivotal players in the dysfunction of cell signaling, structure, and myelination in schizophrenia brains. EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE, v. 265, n. 7, p. 601-612, OCT 2015. Web of Science Citations: 27.
CASSOLI, JULIANA SILVA; GUEST, PAUL C.; MALCHOW, BEREND; SCHMITT, ANDREA; FALKAI, PETER; MARTINS-DE-SOUZA, DANIEL. Disturbed macro-connectivity in schizophrenia linked to oligodendrocyte dysfunction: from structural findings to molecules. NPJ SCHIZOPHRENIA, v. 1, 2015. Web of Science Citations: 18.
MARTINS-DE-SOUZA, DANIEL; SOLARIA, FIORELLA A.; GUEST, PAUL C.; ZAHEDI, RENE P.; STEINER, JOHANN. Biological pathways modulated by antipsychotics in the blood plasma of schizophrenia patients and their association to a clinical response. NPJ SCHIZOPHRENIA, v. 1, 2015. Web of Science Citations: 6.
NASCIMENTO, JULIANA M.; MARTINS-DE-SOUZA, DANIEL. The proteome of schizophrenia. NPJ SCHIZOPHRENIA, v. 1, 2015. Web of Science Citations: 33.

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