Characterization of monocytes and macrophages activation states present in the mouse pubic symphysis remodeling at late pregnancy and postpartum.

• Grant number: 15/23616-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: August 31, 2018
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Paulo Pinto Joazeiro
• Grantee: Paulo Pinto Joazeiro
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Jörg Kobarg ; Sílvio Roberto Consonni

Abstract

Like others birth canal musculoskeletal elements, the mice Pubic Symphysis (PS) presents a complex tissue remodeling during the first pregnancy, parturition, and postpartum. At late pregnancy, this dynamic tissue remodeling of birth canal musculoskeletal elements has been associated with increased leukocyte proinflammatory processes.The adaptive remodeling of mouse PS to progressive biomechanical demands during pregnancy depends on cell-mediated and matrix-associated changes. In short terms, changes in composition and assemblies of proteoglycans, collagen and elastin fibers architecture and also increased deposition of high molecular weight hyaluronan. Concerning the leukocyte recruitment to the mouse PS, in response to environmental changes during late pregnancy, our previous results showed that monocytes and macrophages are the main transient cell subsets.Based on our previous results and recent progress on gene expression patterns among mouse macrophages in tissue homeostasis and repair, we propose this study to characterize spectra of activation states of monocytes and macrophages present in PS physiological remodeling during pregnancy and regenerative postpartum recovery.Results of this study may indicate the existence of alternative mechanisms responsible for transitory macrophage contribution to PS remodeling and recovery. This contribution may be useful for news and proof-of-concept studies for joint and pelvic griddle repair. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASTELUCCI, BIANCA G.; CONSONNI, SILVIO R.; ROSA, VIVIANE S.; JOAZEIRO, PAULO P.. Recruitment of monocytes and mature macrophages in mouse pubic symphysis relaxation during pregnancy and postpartum recovery. BIOLOGY OF REPRODUCTION, v. 101, n. 2, p. 466-477, AUG 2019. (12/25038-8, 15/23616-2)

CASTELUCCI, BIANCA GAZIERI; CONSONNI, SILVIO ROBERTO; ROSA, VIVIANE SOUZA; SENSIATE, LUCIMARA APARECIDA; RUGNO DELATTI, PAULA CRISTINA; ALVARES, LUCIA ELVIRA; JOAZEIRO, PAULO PINTO. Time-dependent regulation of morphological changes and cartilage differentiation markers in the mouse pubic symphysis during pregnancy and postpartum recovery. PLoS One, v. 13, n. 4, APR 5 2018. (12/25038-8, 15/23616-2)

CASTELUCCI, B. G.; PEREIRA, A. H. M.; FIORAMONTE, M.; CARAZZOLLE, M. F.; DE OLIVEIRA, P. S. L.; FRANCHINI, K. G.; KOBARG, J.; MARTINS-DE-SOUZA, D.; JOAZEIRO, P. P.; CONSONNI, S. R.. Evidence of macrophage modulation in the mouse pubic symphysis remodeling during the end of first pregnancy and postpartum. SCIENTIFIC REPORTS, v. 10, n. 1, JUL 24 2020. (15/23616-2, 17/03489-1)