KIR2DL4, KIR3DL2, KIR3DL3, LILRB1 (ILT2) and LILRB2 (ILT4) variability and haplotypes in a Brazilian population sample from the São Paulo state

• Grant number: 17/19223-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2018
• End date: January 31, 2020
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Erick da Cruz Castelli
• Grantee: Erick da Cruz Castelli
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Celso Teixeira Mendes Junior ; Eduardo Antônio Donadi

Abstract

The Leukocyte Receptors Complex (LRC), which comprehends approximately 1Mb of chromosome 19, is comprised by genes that mostly codify molecules of the Immunoglobulin superfamily (Ig). Among these genes, the Immunoglobulin-like Killer Receptors Family (KIR), expressed on Natural Killer (NK) cells and T cytotoxic lymphocytes, and the Leucocyte Immunoglobulin-like Receptors (LILRs), expressed both on myeloid and lymphoid origin cells, are highlighted. The main ligands of these receptors are the class I HLA molecules from the Major Histocompatibility Complex (MHC), coded by the most variable genes of the human genome. Together, these receptor-ligand complexes are responsible for controlling and modulating the immune responses. The inherent variability of KIR2DL4, KIR3DL2, KIR3DL3, LILRB1(ILT2) and LILRB2(ILT4) genes is poorly explored, although some of these genes seem to be very polymorphic, as reported in previous studies that evaluated only some éxons of these genes. There are no studies describing the variability of these genes in Brazil, which has a highly heterogeneous and admixed population, and is considered a great repository of genetic variability as previously reported in studies characterizing the class I MHC genes. In addition, there is no massive sequencing methodology established to explore the complete variability of these genes. Thus, the aim of this study is to elaborate a strategy to evaluate KIR2DL4, KIR3DL2, KIR3DL3, LILRB1(ILT2) and LILRB2 (ILT4) genes using next generation sequencing and to explore the genetic and haplotypic variability of these genes in a Brazilian sample, correlating the data acquired with the existent variability data of the HLA genes previously evaluated in these same samples. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, ANDREIA S.; SONON, PAULIN; PAZ, MICHELLE A.; TOKPLONOU, LEONIDAS; LIMA, THALITTA H. A.; PORTO, IANE O. P.; ANDRADE, HELOISA S.; SILVA, NAYANE DOS S. B.; VEIGA-CASTELLI, LUCIANA C.; OLIVEIRA, MARIA LUIZA G.; et al. Hla-Cgenetic diversity and evolutionary insights in two samples from Brazil and Benin. HLA, AUG 2020. (17/19223-0, 13/17084-2)

DE CASTRO, MATEUS, V; SILVA, MONIZE V. R.; NASLAVSKY, MICHEL S.; SCLIAR, MARILIA O.; NUNES, KELLY; PASSOS-BUENO, MARIA RITA; CASTELLI, ERICK C.; MAGAWA, JHOSIENE Y.; ADAMI, FLAVIA L.; MORETTI, ANA I. S.; et al. The oldest unvaccinated Covid-19 survivors in South America. IMMUNITY & AGEING, v. 19, n. 1, p. 9-pg., 2022-11-16. (14/50890-5, 17/19223-0, 14/50931-3, 13/08028-1)

CASTELLI, ERICK C.; DE ALMEIDA, BIBIANA S.; MUNIZ, YARA C. N.; SILVA, NAYANE S. B.; PASSOS, MARILIA R. S.; SOUZA, ANDREIA S.; PAGE, ABIGAIL E.; DYBLE, MARK; SMITH, DANIEL; AGUILETA, GABRIELA; et al. HLA-G genetic diversity and evolutive aspects in worldwide populations. SCIENTIFIC REPORTS, v. 11, n. 1, NOV 29 2021. (17/19223-0)

CASTELLI, ERICK C.; DE CASTRO, V, MATEUS; NASLAVSKY, MICHEL S.; SCLIAR, MARILIA O.; SILVA, NAYANE S. B.; ANDRADE, HELOISA S.; SOUZA, ANDREIA S.; PEREIRA, RAPHAELA N.; CASTRO, CAMILA F. B.; MENDES-JUNIOR, CELSO T.; et al. MHC Variants Associated With Symptomatic Versus Asymptomatic SARS-CoV-2 Infection in Highly Exposed Individuals. FRONTIERS IN IMMUNOLOGY, v. 12, SEP 28 2021. (13/08028-1, 19/19998-8, 20/09702-1, 17/19223-0, 14/50931-3)

WEISS, EMILIANA; ANDRADE, HELOISA S.; LARA, JULIANA RODRIGUES; SOUZA, ANDREIA S.; PAZ, MICHELLE A.; LIMA, THALITTA H. A.; PORTO, IANE O. P.; S. B. SILVA, NAYANE; CASTRO, CAMILA F. BANNWART; GROTTO, REJANE M. T.; et al. KIR2DL4 genetic diversity in a Brazilian population sample: implications for transcription regulation and protein diversity in samples with different ancestry backgrounds. IMMUNOGENETICS, v. 73, n. 3, FEB 2021. (17/19223-0, 17/05042-4)

GUIMARAES DE OLIVEIRA, MARIA LUIZA; CASTELLI, ERICK C.; VEIGA-CASTELLI, LUCIANA C.; PEREIRA, ALISON E.; MARCORIN, LETICIA; CARRATTO, THASSIA M. T.; SOUZA, ANDREIA S.; ANDRADE, HELOISA S.; SIMOES, AGUINALDO L.; DONADI, EDUARDO A.; et al. Genetic diversity of the LILRB1 and LILRB2 coding regions in an admixed Brazilian population sample. HLA, v. 100, n. 4, p. 24-pg., 2022-07-27. (13/15447-0, 17/19223-0)

SOUZA, ANDREIA S.; SONON, PAULIN; PAZ, MICHELLE A.; TOKPLONOU, LEONIDAS; LIMA, THALITTA H. A.; PORTO, IANE O. P.; ANDRADE, HELOISA S.; SILVA, NAYANE DOS S. B.; VEIGA-CASTELLI, LUCIANA C.; OLIVEIRA, MARIA LUIZA G.; et al. Hla-C genetic diversity and evolutionary insights in two samples from Brazil and Benin. HLA, v. 96, n. 4, p. 19-pg., 2020-08-24. (17/19223-0, 13/17084-2)

SILVA, NAYANE DOS SANTOS BRITO; SOUZA, ANDREIA DA SILVA; ANDRADE, HELOISA DE SOUZA; PEREIRA, RAPHAELA NETO; CASTRO, CAMILA FERREIRA BANNWART; VINCE, NICOLAS; LIMOU, SOPHIE; NASLAVSKY, MICHEL SATYA; ZATZ, MAYANA; DUARTE, YEDA APARECIDA DE OLIVEIRA; et al. Immunogenetics of HLA-B: SNP, allele, and haplotype diversity in populations from different continents and ancestry backgrounds. HLA, v. 101, n. 6, p. 13-pg., 2023-04-02. (17/19223-0, 13/17084-2, 21/02815-8)

CASTELLI, ERICK C.; DE CASTRO, MATEUS V.; NASLAVSKY, MICHEL S.; SCLIAR, MARILIA O.; SILVA, NAYANE S. B.; PEREIRA, RAPHAELA N.; CIRIACO, VIVIANE A. O.; CASTRO, CAMILA F. B.; MENDES-JUNIOR, CELSO T.; SILVEIRA, ETIELE DE S.; et al. MUC22, HLA-A, and HLA-DOB variants and COVID-19 in resilient super-agers from Brazil. FRONTIERS IN IMMUNOLOGY, v. 13, p. 15-pg., 2022-10-25. (14/50931-3, 20/09702-1, 17/19223-0, 19/19998-8, 13/08028-1)

TELLES CARRATTO, THASSIA MAYRA; MARCORIN, LETICIA; DEBORTOLI, GUILHERME; HUNEMEIER, TABITA; NORTON, HEATHER; PARRA, ESTEBAN JUAN; CASTELLI, ERICK C.; MENDES-JUNIOR, CELSO TEIXEIRA. Insights on hair, skin and eye color of ancient and contemporary Native Americans. FORENSIC SCIENCE INTERNATIONAL-GENETICS, v. 48, p. 9-pg., 2020-09-01. (17/19223-0, 13/15447-0)

CASTELLI, E. C.; OLIVEIRA, M. G.; VEIGA-CASTELLI, L. C.; SOUZA, A. S.; LIMA, T. H. A.; ANDRADE, H. S.; WEISS, E.; PASSOS, M. R. S.; MARCORIN, L.; DEBORTOLI, G.; et al. A deep characterization of the LILRB2/ILT4 genetic variability in Brazilian samples with different ancestry compositions. European Journal of Human Genetics, v. 27, p. 1-pg., 2019-10-01. (17/19223-0)

NASLAVSKY, MICHEL S.; SCLIAR, MARILIA O.; YAMAMOTO, GUILHERME L.; WANG, JAQUELINE YU TING; ZVERINOVA, STEPANKA; KARP, TATIANA; NUNES, KELLY; MAGLIOCCO CERONI, JOSE RICARDO; DE CARVALHO, DIEGO LIMA; DA SILVA SIMOES, CARLOS EDUARDO; et al. Whole-genome sequencing of 1,171 elderly admixed individuals from Sao Paulo, Brazil. NATURE COMMUNICATIONS, v. 13, n. 1, p. 11-pg., 2022-03-04. (12/24731-1, 20/02413-4, 14/50649-6, 13/08028-1, 18/15579-8, 15/25020-0, 17/19223-0, 13/17084-2)