MATRIX EFFECT ON VEHICULATION AND FUNCTIONALITY OF PRE AND PROBIOTICS: STUDY IN A DYNAMIC COLONIC MODEL AND WITH VOLUNTEERS FROM THE PREPARATORY SCHOOL OF ARMY CADETS FROM CAMPINAS

• Grant number: 17/25007-9
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2018
• End date: March 31, 2020
• Field of knowledge: Agronomical Sciences - Food Science and Technology
• Principal Investigator: Adriane Elisabete Antunes de Moraes
• Grantee: Adriane Elisabete Antunes de Moraes
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Ângela Giovana Batista ; Darlila Aparecida Gallina ; Fernando Moreira Simabuco ; Glaucia Maria Pastore ; Katia Sivieri ; Mário Roberto Maróstica Junior ; Rosangela dos Santos
• Associated scholarship(s): 18/18366-5 - Culture-based and non-culture based techniques for analysis of probiotics and metabolites of the microbiota, BP.TT

Abstract

The state of the art on pre and probiotic does not present a definitive answer on the best way of delivery of them. The objective of the present study is to compare one food matriz (ice cream obtained on a semi-industrial scale) and a lyophilized food supplement containing the same amounts of inulin, Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12. Static simulated digestion (metabolic bath) and dynamics (simulator of the human gastrointestinal tract) will be employed. The viability, metabolic state, cellular morphology and viability (by techniques based or not on cultivation) of the probiotics in ice cream and lyophilized food supplement will be obtained. At the final step of the research a double blind clinical study will be made to evaluate the effect of supplementation of volunteers of the Preparatory School of Cadets of Campinas / SP in parameters of microbiota, gut-brain axis and general health. All variables will be analyzed for their distribution. The results of the microbiota analysis will undergo bioinformatics treatment. Variables involving humans will be compared by paired tests (paired t-student or Wilcoxon) before and after the proposed intervention. Correlation and association tests will be performed to verify the relationship of the variables. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, VIVIAN CRISTINA DA CRUZ; DUQUE, ANA LUIZA ROCHA FARIA; FINO, LUCIANA DE CARVALHO; SIMABUCO, FERNANDO MOREIRA; SARTORATTO, ADILSON; CABRAL, LUCELIA; NORONHA, MELLINE FONTES; SIVIERI, KATIA; ANTUNES, ADRIANE ELISABETE COSTA. Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics. BRITISH JOURNAL OF NUTRITION, v. 124, n. 1, p. 57-68, JUL 14 2020. (17/25007-9)

FREITAS, SAMARA M.; FRANCO, BEATRIZ; SARAGIOTTO, GIULIO; MORAIS, MILCA A.; SIMABUCO, FERNANDO M.; CUNHA, DIOGO T.; ESTEVES, ANDREA M.; ANTUNES, ADRIANE E. C.. Effect of a probiotic fermented milk supplementation on behavior and sleep. NUTRITIONAL NEUROSCIENCE, v. N/A, p. 13-pg., 2023-07-27. (17/25007-9)

DA CRUZ RODRIGUES, VIVIAN CRISTINA; SALVINO DA SILVA, LUIZ GUILHERME; SIMABUCO, FERNANDO MOREIRA; VENEMA, KOEN; COSTA ANTUNES, ADRIANE ELISABETE. Survival, metabolic status and cellular morphology of probiotics in dairy products and dietary supplement after simulated digestion. Journal of Functional Foods, v. 55, p. 126-134, APR 2019. (17/25007-9)

VALLE, MARIA C. P. R.; VIEIRA, ISABEL A.; FINO, LUCIANA C.; GALLINA, DARLILA A.; ESTEVES, ANDREA M.; DA CUNHA, DIOGO T.; CABRAL, LUCELIA; BENATTI, FABIANA B.; MAROSTICA JUNIOR, MARIO R.; BATISTA, ANGELA G.; et al. Immune status, well-being and gut microbiota in military supplemented with synbiotic ice cream and submitted to field training: a randomised clinical trial. BRITISH JOURNAL OF NUTRITION, v. 126, n. 12, p. 1794-1808, DEC 28 2021. (18/18366-5, 15/50333-1, 17/25007-9, 18/11069-5, 15/13320-9)