Fixed bed bioreactors to solid state fermentation: scale-up to cellullase fungal production

• Grant number: 11/07453-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2011
• End date: April 30, 2015
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal Investigator: João Cláudio Thoméo
• Grantee: Fernanda Perpétua Casciatori
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 12/13939-0 - Modeling of heat transfer and moisture migration in packed bed solid state fermentation bioreactors for fungal cellulase production using agro-industrial solid by-products, BE.EP.DR

Abstract

The objective is to produce cellulases by solid state fermentation (SSF) in fixed bed bioreactor, through various strategies to expand the scale, and by using agro-industrial residues as substrates. At this time of discussion of Brazil's energy matrix, the cellulases are relevant for the hydrolysis of lignocellulosic materials aimed at producing second-generation ethanol. Although the FES produce high concentrations of enzymes, this technique lacks technological expertise that enables its application in large scale. To contribute to the advancement in the area, this project involves stages of experimentation and simulation. From a bench scale fermenter, several alternatives will be used to expand the scale to overcome the difficulties inherent in the operation of this class of bioreactors, namely the separation of moisture and thermal heterogeneity, culminating in a pilot scale fermenter of 400 L. The fungi to be used will be the thermophilic Myceliophtora sp. and mesophilic Aspergillus niger and the agro-industrial residues will be bagasse from sugar cane and wheat bran. The use of fungi with different characteristics lends itself to assess whether the alternative technologies have found wide application spectrum. To simulate the process, you will need to establish experimentally the growth kinetics of microorganisms. The models to be applied will be the most general possible, predicting the temperature variation as a function of time and space, which will be compared with data from the trials. The project falls under the theme of the FAPESP Research Program on Bioenergy (BIOEN) and the Center for Bioenergy Research Paulista, and is being proposed along the line of research Process Engineering for the Food Industry, the Post-Graduate Engineering and Food Science, Institute of Biosciences, Language, and Science. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASCIATORI, FERNANDA PERPETUA; LAURENTINO, CARMEM LUCIA; ZANELATO, ALEX IZUKA; THOMEO, JOAO CLAUDIO. Hygroscopic properties of solid agro-industrial by-products used in solid-state fermentation. INDUSTRIAL CROPS AND PRODUCTS, v. 64, p. 114-123, FEB 2015. (11/07453-5)

REZENDES TADA, ERIKA FERNANDA; BUECK, ANDREAS; CASCIATORI, FERNANDA PERPETUA; TSOTSAS, EVANGELOS; CLAUDIO THOMEO, JOAO. Investigation of heat transfer in partially filled horizontal drums. CHEMICAL ENGINEERING JOURNAL, v. 316, p. 988-1003, MAY 15 2017. (12/13939-0, 14/23453-3, 14/25183-3, 11/07453-5, 15/00362-5)

CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO. Heat transfer in packed-beds of agricultural waste with low rates of air flow applicable to solid-state fermentation. Chemical Engineering Science, v. 188, p. 97-111, OCT 12 2018. (14/25183-3, 12/13939-0, 14/23453-3, 18/00996-2, 11/07453-5)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, 1, p. 2097-2115, 2020. (13/01756-1, 18/00996-2, 17/16482-5, 11/07453-5, 10/12624-0, 11/21239-6)

CASCIATORI, FERNANDA PERPETUA; LAURENTINO, CARMEM LUCIA; TABOGA, SEBASTIAO ROBERTO; CASCIATORI, PRISCILA APARECIDA; THOMEO, JOAO CLAUDIO. Structural properties of beds packed with agro-industrial solid by-products applicable for solid-state fermentation: Experimental data and effects on process performance. CHEMICAL ENGINEERING JOURNAL, v. 255, p. 214-224, NOV 1 2014. (11/07453-5)

CASCIATORI, FERNANDA PERPETUA; LAURENTINO, CARMEM LUCIA; LEAL DA COSTA, KAREN KRISTHINE; CASCIATORI, PRISCILA APARECIDA; THOMEO, JOAO CLAUDIO. HYGROSCOPIC PROPERTIES OF ORANGE PULP AND PEEL. JOURNAL OF FOOD PROCESS ENGINEERING, v. 36, n. 6, p. 803-810, DEC 2013. (12/13939-0, 11/07453-5, 10/12624-0, 11/21239-6)

CASCIATORI, FERNANDA PERPETUA; BUECK, ANDREAS; THOMEO, JOAO CLAUDIO; TSOTSAS, EVANGELOS. Two-phase and two-dimensional model describing heat and water transfer during solid-state fermentation within a packed-bed bioreactor. CHEMICAL ENGINEERING JOURNAL, v. 287, p. 103-116, MAR 1 2016. (11/07453-5, 12/13939-0, 14/25183-3)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, p. 19-pg., 2020-01-01. (17/16482-5, 11/07453-5, 13/01756-1, 10/12624-0, 18/00996-2, 11/21239-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CASCIATORI, Fernanda Perpétua. Produção de celulases fúngicas por fermentação em estado sólido: ampliação de escala de biorreatores de leito fixo. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.