Molecular investigation of Klebsiella pneumoniae strains and plasmids carrying beta-lactamases encoding genes

• Grant number: 11/08892-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2011
• End date: May 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ana Lúcia da Costa Darini
• Grantee: Leonardo Neves de Andrade
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 13/26188-6 - Virulence factors and intraclonal diversity of ESBL and carbapenemase-producing Klebsiella pneumoniae and Escherichia coli clinical isolates, BE.EP.PD

Abstract

Beta-lactamases-producing enterobacteria are a worldwide problem, observed mainly in the last two decades with the pandemic of beta-lactamases CTX-M and most recently the dissemination of KPC and emergence of NDM carbapenemases. In Brazil, few studies report with detail the molecular epidemiology of bacterial strains prevalent and/or epidemic plasmids that are involved in the spread of resistance. In this context, the aim of this project is to investigate K. pneumoniae isolates and plasmids carrying beta-lactamases encoding genes in the northeast region of São Paulo State - Brazil. Thus, isolates of broad-spectrum beta-lactam-resistant K. pneumoniae will be investigated. Beta-lactamases encoding genes will be investigated, especially those commonly carried by plasmids. The bacterial strains will be defined considering the macrorestriction profile by pulsed-field gel electrophoresis and, according to the sequence type, by Multilocus Sequence Typing. The characterization of plasmids will be accomplished by typing of incompatibility groups and hybridization. This knowledge will be fundamental in near future since new strategies can be formulated to control antibiotic resistance, more incisive and effective, directly in the chain of resistance genes spread.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDRADE, LEONARDO NEVES; NOVAIS, ANGELA; MARCATO STEGANI, LENITA MARIA; FERREIRA, JOSEANE CRISTINA; RODRIGUES, CARLA; COSTA DARINI, ANA LUCIA; PEIXE, LUISA. Virulence genes, capsular and plasmid types of multidrug-resistant CTXM(-2,-8,-15) and KPC-2-producing Klebsiella pneumoniae isolates from four major hospitals in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 91, n. 2, p. 164-168, JUN 2018. (12/14740-3, 11/08892-2, 13/26188-6)

GASPAR, GILBERTO GAMBERO; BELLISSIMO-RODRIGUES, FERNANDO; DE ANDRADE, LEONARDO NEVES; DARINI, ANA LUCIA; MARTINEZ, ROBERTO. Induction and nosocomial dissemination of carbapenem and polymyxin-resistant Klebsiella pneumoniae. Revista da Sociedade Brasileira de Medicina Tropical, v. 48, n. 4, p. 483-487, JUL-AUG 2015. (11/08892-2)

ANDRADE, LEONARDO NEVES; VITALI, LUCIA; GASPAR, GILBERTO GAMBERO; BELLISSIMO-RODRIGUES, FERNANDO; MARTINEZ, ROBERTO; COSTA DARINI, ANA LUCIA. Expansion and Evolution of a Virulent, Extensively Drug-Resistant (Polymyxin B-Resistant), QnrS1-, CTX-M-2-, and KPC-2-Producing Klebsiella pneumoniae ST11 International High-Risk Clone. Journal of Clinical Microbiology, v. 52, n. 7, p. 2530-2535, JUL 2014. (12/14740-3, 11/08892-2)

FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; ANDRADE, LEONARDO NEVES; BERCHIERI JUNIOR, ANGELO; COSTA DARINI, ANA LUCIA. Evaluation and characterization of plasmids carrying CTX-M genes in a non-clonal population of multidrug-resistant Enterobacteriaceae isolated from poultry in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 85, n. 4, p. 444-448, AUG 2016. (14/14494-8, 11/08892-2, 12/24017-7)