Immobilization and stabilization of lipases from Candida antarctida: fraction b and its application in biocatalysis

• Grant number: 13/00530-0
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: September 01, 2013
• End date: February 28, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Mauricio Boscolo
• Grantee: José Carlos Quilles Junior
• Supervisor: José M. Guisan
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Institution abroad: Universidad Autónoma de Madrid (UAM), Spain
• Associated to the scholarship: 12/09054-3 - Immobilization of lipases on agarose and chitosan and application in biocatalysis, BP.MS

Abstract

The project stage of research abroad aims to improve work techniques immobilization and stabilization of lipases and their application in biocatalysis of reactions of hydrolysis and transesterification of vegetable oil. Lipases from Candida antarctica - fraction B will be immobilized by different methods of immobilization (covalent bonding and hydrophobic interaction) and its stability will be studied in ethanol and its thermostability. The immobilized enzymes are applied in reactions of racemic mixtures to try to distinguish the enantiomer of interest, on hydrolysis of fish oil to produce free fatty acids and ethanolysis reaction on vegetable oil for the synthesis of ethyl biodiesel. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORGES, JANAINA PIRES; QUILLES JUNIOR, JOSE CARLOS; MORENO-PEREZ, SONIA; FERNANDEZ-LORENTE, GLORIA; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO; BOCCHINI, DANIELA ALONSO; GUISAN, JOSE MANUEL. Ethyl esters production catalyzed by immobilized lipases is influenced byn-hexaneandter-amyl alcohol as organic solvents. Bioprocess and Biosystems Engineering, JUN 2020. (13/00530-0, 12/09054-3)

QUILLES, J. C. J.; BRITO, R. R.; BORGES, J. P.; ARAGON, CC.; FERNANDEZ-LORENTED, G.; BOCCHINI-MARTINS, D. A.; GOMES, E.; DA SILVA, R.; BOSCOLO, M.; GUISAN, J. M.. Modulation of the activity and selectivity of the immobilized lipases by surfactants and solvents. Biochemical Engineering Journal, v. 93, p. 274-280, JAN 15 2015. (13/00530-0)

QUILLES JUNIOR, JOSE CARLOS; FERRAREZI, ANA LUCIA; BORGES, JANAINA PIRES; BRITO, RAFAELA RODRIGUES; GOMES, ELENI; DA SILVA, ROBERTO; MANUEL GUISAN, JOSE; BOSCOLO, MAURICIO. Hydrophobic adsorption in ionic medium improves the catalytic properties of lipases applied in the triacylglycerol hydrolysis by synergism. Bioprocess and Biosystems Engineering, v. 39, n. 12, p. 1933-1943, DEC 2016. (13/00530-0, 12/09054-3)

BORGES, JANAINA PIRES; QUILLES JUNIOR, JOSE CARLOS; MORENO-PEREZ, SONIA; FERNANDEZ-LORENTE, GLORIA; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO; BOCCHINI, DANIELA ALONSO; GUISAN, JOSE MANUEL. Ethyl esters production catalyzed by immobilized lipases is influenced byn-hexaneandter-amyl alcohol as organic solvents. Bioprocess and Biosystems Engineering, v. 43, n. 11, p. 9-pg., 2020-06-27. (13/00530-0, 12/09054-3)