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Study of the effect of chronic stimulation cholinergic with pyridostigmine bromide on cardiovascular autonomic adaptations and cardiac morphofunctional induced by physical training aerobic in spontaneously hipertensive rats

Grant number: 14/00940-6
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2014
Effective date (End): April 30, 2016
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Hugo Celso Dutra de Souza
Grantee:Camila Balsamo Gardim
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The cholinergic stimulation through the inhibition of acetylcholinesterase (AChE) produces effects on the cardiac autonomic control that resemble those obtained through the aerobic physical training, both in humans and in experimental models. These effects are characterized mainly by significant increase in Heart Rate Variability (HRV) and in baroreflex sensitivity. However, up until now, no study has addressed the morphofunctional and autonomic effects cardiac stimulation of chronic cholinergic hypertension, nor the association of same with the aerobic physical training. In this context, our objective is to investigate the effect of chronic cholinergic stimulation, by means of chronic treatment with an inhibitor of acetylcholinesterase (pyridostigmine bromide) in three different daily dosages (5mg, 10mg and 20mg/kg), on various parameters and cardiac autonomic morphofunctional in Spontaneously Hypertensive Rats (SHR), both sedentary, as submitted to aerobic physical training. The choice of this experimental model is due to the fact that this lineage of rats, SHR, is classically known due to cardiac morphofunctional changes deleterious, associated with injury in cardiovascular autonomic control. For both, SHR rats (N= 96) will be divided into two groups, sedentary (N= 48) and trained (N= 48). Each group will be divided into four smaller groups (N= 12); control SHR treated with placebo in the drinking water; and SHR groups treated with pyridostigmine bromide, also dissolved in drinking water, in three different dosages, 1mg, 10mg and 20mg/kg. All groups will have the cardiovascular autonomic control assessed by means of the baroreflex sensitivity, cardiac autonomic tonus, analysis of heart rate variability and blood pressure and catecholamine levels. While the morphofunctional values shall be obtained by means of two-dimensional echocardiography and histological analysis of cardiac tissue. (AU)

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GARDIM, Camila Balsamo. Study of chronic cholinergic stimulation with pyridostigmine bromide effect on hemodynamic changes, cardiac autonomic and morphofunctional induced by aerobic exercise in spontaneously hypertensive rats (SHR). 2016. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto Ribeirão Preto.

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