Development of a vaccine formulation against dengue based on the nonstructural protein 1 (NS1) administered by the intradermal route

• Grant number: 13/26942-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2014
• End date: May 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Luis Carlos de Souza Ferreira
• Grantee: Lennon Ramos Pereira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In this project we intend to develop and characterize a prophylactic vaccine strategy against dengue virus based on NS1 protein in combination with adjuvant. To achieve this, the recombinant NS1 protein DENV-2 and a truncated form of NS1 DENV 2 (DeltaC-NS1) will be expressed in E. coli and purified by metal affinity chromatography. Vaccine formulations will be prepared based on the NS1 and DeltaC-NS1 and administered as an antigen in BALB/c mice by intradermal route, in combination with the adjuvant poly (I:C). The cellular immune responses induced by anti-NS1 tested vaccine formulations will be monitored by testing as ICS (Intracellular Staining Citokine), ELISPOT (Enzyme-Linked Immunosorbent Spot) and measurement of cytotoxic activity in vivo. The humoral response induced anti-NS1 will be monitored by measurement of total IgG and IgG subclass anti-NS1 by ELISA. To evaluate the protective capacity conferred by the vaccine regimen, the mice will be challenged with a lethal dose of dengue virus. Moreover, the potential autoimmune responses induced by immunization with NS1 and DeltaC-NS1 will be measured by cross-reactivity with human serum albumin and human platelets in ELISA assays. Thus, at the end of the work, we expect to generate results and knowledge that will contribute to the development of an effective vaccine strategy against dengue virus. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, LENNON RAMOS; VICENTIN, ELAINE CRISTINA MATOS; PEREIRA, SARA ARAUJO; MAEDA, DENICAR LINA NASCIMENTO FABRIS; ALVES, RUBENS PRINCE DOS SANTOS; ANDREATA-SANTOS, ROBERT; SOUSA, FRANCIELLE TRAMONTINI GOMES DE; YAMAMOTO, MARCIO MASSAO; CASTRO-AMARANTE, MARIA FERNANDA; FAVARO, MARIANNA TEIXEIRA DE PINHO; et al. Intradermal Delivery of Dendritic Cell-Targeting Chimeric mAbs Genetically Fused to Type 2 Dengue Virus Nonstructural Protein 1. VACCINES, v. 8, n. 4, DEC 2020. (13/26942-2, 14/04303-0, 16/05570-8, 13/01702-9, 13/11442-4, 14/17595-0, 13/01690-0)

OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; MENDES, ERICA A.; LADNER, JASON T.; ANDREATA-SANTOS, ROBERT; ARAUJO, DANIELLE B.; BOTOSSO, VIVIANE F.; PAOLA, NICHOLAS D.; NETO, DANIEL F. L.; CUNHA, MARIELTON P.; et al. Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses-Basel, v. 10, n. 11, NOV 2018. (13/07467-1, 14/17766-9, 14/27228-4, 14/16333-1, 16/08727-5, 15/25643-7, 14/03911-7, 16/08204-2, 16/20045-7, 13/26942-2, 16/03605-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PEREIRA, Lennon Ramos. Development of vaccines formulations against dengue based on the nonstructural protein 1 (NS1) administered by the intradermal route.. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.