Extraction of espilantol in the context of green chemistry and its application in the treatment of oral mucositis

• Grant number: 14/06461-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2014
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Pharmacy
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Rodney Alexandre Ferreira Rodrigues
• Grantee: Verônica Santana de Freitas Blanco
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The extraction of compounds from plants is usually associated with the use of organic solvents. As a consequence there is the generation of industrial waste causing an environmental impact and high operational cost. For this reason, the use of processes that eliminate the use of organic solvents, such as supercritical extraction employing carbon dioxide it is of great interest. Furthermore, the efficacy, safety and quality in the isolation of bioactive compounds by supercritical extraction technique has been proven experimentally. Spilanthol, an alkylamide found in Spilanthes acmella L. Murray, has several pharmacological properties such as anti - inflammatory, anesthetic and antioxidant activity. These properties may be of great interest in the treatment of oral mucositis, a typical side effect due to the treatment of cancer in patients undergoing radiation and chemotherapy. The aim of this work is to isolate this compound by means of supercritical extraction and evaluate their anti - inflammatory activity through experimental models as well as incorporation in oral formulation as a component in the treatment of mucositis. With this, we will study the possible mechanisms involved in the anti - inflammatory activity through in vitro experiments and also anti - inflammatory and antinociceptive activity in vivo models of paw edema induced by carrageenan and gastrointestinal mucositis, with subsequent histopathological analysis and in vitro mediators involved in the inflammatory process. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALONSO, ISABELLA G.; YAMANE, LAIS T.; DE FREITAS-BLANCO, VERONICA S.; NOVAES, LUIZ F. T.; FRANZ-MONTAN, MICHELLE; DE PAULA, ENEIDA; RODRIGUES, MARILI V. N.; RODRIGUES, RODNEY A. F.; PASTRE, JULIO C.. A new approach for the total synthesis of spilanthol and analogue with improved anesthetic activity. Tetrahedron, v. 74, n. 38, p. 5192-5199, SEP 20 2018. (15/08199-6, 14/06461-2, 14/25770-6, 14/16186-9, 14/26378-2)

DE FREITAS BLANCO, VERONICA SANTANA; MICHALAK, BARBARA; MACCARI ZELIOLI, ICARO AUGUSTO; SANTOS DE OLIVEIRA, ADRIANA DA SILVA; NOVA RODRIGUES, MARILI VILLA; FERREIRA, ANTONIO GILBERTO; GARCIA, VERA LUCIA; CABRAL, FERNANDO ANTONIO; KISS, ANNA KAROLINA; FERREIRA RODRIGUES, RODNEY ALEXANDRE. Isolation of spilanthol from Acmella oleracea based on Green Chemistry and evaluation of its in vitro anti-inflammatory activity. JOURNAL OF SUPERCRITICAL FLUIDS, v. 140, p. 372-379, OCT 2018. (14/06461-2, 15/09644-3, 14/07225-0, 14/16186-9)

DE FREITAS-BLANCO, VERONICA SANTANA; MONTEIRO, KARIN MAIA; DE OLIVEIRA, PATRICIA ROSA; SOUZA DE OLIVEIRA, ELLEN CRISTINA; DE OLIVEIRA BRAGA, LUCIA ELAINE; DE CARVALHO, JOAO ERNESTO; FERREIRA RODRIGUES, RODNEY ALEXANDRE. Spilanthol, the Principal Alkylamide from Acmella oleracea, Attenuates 5-Fluorouracil-Induced Intestinal Mucositis in Mice. Planta Medica, v. 85, n. 3, p. 203-209, FEB 2019. (14/16186-9, 14/06461-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BLANCO, Verônica Santana de Freitas. Extraction of spilanthol in the context of green chemistry and its application in the treatment of oral mucositis. 2018. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.