Visualization and quantification of Fusarium oxysporum f. sp. phaseoli in four varieties of bean plants (Phaseolus vulgaris L.)

• Grant number: 16/00359-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2016
• End date: April 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Juliane Karine Ishida
• Grantee: Lucas Roberto de Oliveira
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Brazil is the largest worldwide producer of common bean (Phaseolus vulgaris L.). However, the strategies to combat pests that compromise the crop production are still incipient. One of them is the fungus Fusarium oxysporum f. sp. phaseoli (FOP). FOP causes a disease known as "fusarium wilt" which attacks the plant through its roots, penetrating toward cortical cells to vascular system. The fungi block the transport of nutrients to aerial part of the plant, reducing the seed production and causing precocious senescence of the leaves. The objective of this project is to understand the temporal-spatial development of fungal infection in four varieties of common beans with different levels of resistance against FOP. For that, visualization technic will be applied using the histochemical dyes 5-bromo-4-chloro-3-indoxyl-2-acetamido-2-deoxy-²-D-glucopyranoside which recognize components of fungal cell wall. Furthermore, the fungal biomass will be quantified along the plant infection by optics density measurement highlighted by the dye 4-nitrophenyl-±-L-arabinofuranoside (NP-ARA), and through quantitative chain reaction of the polimerase (qPCR), applying fungal genomic genes (ftf2 and ef1-±). Once the temporal-spatial progress of fungal infection is understood, I will explore the microbial diversity of Amazonian dark soil to search for microorganisms able to alter fungal progression into plant tissue. The results from these observations will be possible to shed a light on elucidation of how FOP interacts and develops in different varieties of common bean plant. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ISHIDA, JULIANE KARINE; CALDAS, DANIELLE G. G.; OLIVEIRA, LUCAS ROBERTO; FREDERICI, GABRIELA CAMPOS; PEREIRA LEITE, LUCAS MARGATO; MUI, TSAI SIU. Genome-wide characterization of the NRAMP gene family in Phaseolus vulgaris provides insights into functional implications during common bean development. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 4, p. 820-833, OCT-DEC 2018. (16/00385-8, 13/06301-2, 16/00359-7)

ISHIDA, JULIANE KARINE; CALDAS, DANIELLE G. G.; OLIVEIRA, LUCAS ROBERTO; FREDERICI, GABRIELA CAMPOS; PEREIRA LEITE, LUCAS MARGATO; MUI, TSAI SIU. Genome-wide characterization of the NRAMP gene family in Phaseolus vulgaris provides insights into functional implications during common bean development. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 4, p. 14-pg., 2018-10-01. (13/06301-2, 16/00359-7, 16/00385-8)