Local toxic effects evaluation in epidermis due dyes exposure used in hair dyes

• Grant number: 16/15660-4
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: November 01, 2016
• End date: January 31, 2020
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Danielle Palma de Oliveira
• Grantee: Camila Alessandra Mini
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Too much used animals in research starting about 3R's Concept what means Reduction, Refinement and Replacement, carrying the trend for global acceptance and many laboratories creation. About the Brazil, alternative methods proposed from European Union occurred in 2014 with seventeen methods recognized. The cosmetic industries have using some methods in 3D cultures due similarity to human tissue and more efficiency than conventional methods. The cosmetics like hair dyes are used worldwide, only Brazil a quarter of population dyed at least once. The International Agency of Research on Cancer (IARC) considers having substances in hair dyes capable cause toxic effects in humans. Preliminary assays with dyes Basic Blue 99 (BB99) and Basic Red 51 (BR51) proved cytotoxic effects in monolayer cultures of keratinocytes like as 3D epidermis, in lower concentrations than maximum recommended to European Scientific Committee. The objective in this study, is the local effects evaluation provoked by dyes and involved mechanism of toxicity evaluation in immortalized keratinocytes (HaCaT) monolayer culture, equivalent and reconstructed epidermis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MINI, C. A.; DORTA, D. J.; MARIA-ENGLER, S. S.; OLIVEIRA, D. P.. Immortalized equivalent human epidermis as a platform to evaluation hair dyes toxicity: Efficiency comparison between 3D and monolayer culture. Chemico-Biological Interactions, v. 330, OCT 1 2020. (16/15660-4)

MINI, C. A.; DREOSSI, S. A. C.; ABE, F. R.; MARIA-ENGLER, S. S.; OLIVEIRA, D. P.. Immortalized keratinocytes cells generates an effective model of Epidermal Human Equivalent for irritation and corrosion tests. TOXICOLOGY IN VITRO, v. 71, MAR 2021. (16/15660-4, 17/04926-6, 19/14527-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MINI, Camila Alessandra. Establishment of a tested 3D epidermis model of an immortalized cells as a platform for assessing dermal toxicity induced by hair dyes. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.