Microfluidics for 3D h-ADMCs culture in hyaluronic acid free or structured in sponges: chemotaxis and chemokinesis in response to growth factors gradients from L-PRP

• Grant number: 17/02913-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 01, 2017
• End date: June 01, 2018
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Maria Helena Andrade Santana
• Grantee: Bruna Alice Gomes de Melo
• Supervisor: Ali Khademhosseini
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: Harvard-MIT Program in Health Sciences and Technology (HST) , United States
• Associated to the scholarship: 15/23134-8 - Influence of the association between platelet-rich plasma and hyaluronic acid on the release of growth factors, cellular proliferation and osteogenic/ chondrogenic differentiation, BP.DR

Abstract

The platelet-rich plasma (PRP) is an autologous product obtained from the whole blood, which are able to stimulate tissue regeneration. The PRP is composed of platelets, which are rich in growth factors (GFs) and leucocytes (called L-PRP) that have specific functions on cellular process, such as angiogenesis, mitogenesis, chemotaxis, proliferation and differentiation. GFs are one of the three components required for cell proliferation, along with the progenitor cells that are responsible for the genic capacity, and scaffolds, which are the matrix that conducts the proliferation. Activated L-PRP are able to form fibrin networks, which are natural scaffolds. Hyaluronic acid (HA) is a glycosaminoglycan found in the extracellular matrix (ECM) and has been used combined with activated L-PRP as scaffold for cell growth, promoting a synergistic effect on bone regeneration, with successful results in clinical studies. However, there is a lack of fundamental and preclinical studies involving the interactions between cells, scaffolds and the GFs released from L-PRP. Microfluidics is a technology that has been recently used for 3D cell culture-based assays because of its many advantages over conventional culture: the creation of an in vitro microenvironment that mimics the in vivo model; the ability to precisely manipulate a tiny fluid for medium perfusion; the quantification of cell behavior in response to gradients, and the low consumption of research resources due to small volumes required. The main goal of this project is the use of growth factors released from PRP for microfluidics mesenchymal stem cells from adipose tissue from human (h-AdMCs) culture assays, and osteogenic/chondrogenic differentiation in the presence of a HA free (low and high molecular weights) or structured in sponges. Besides, we aim to perform chemotaxis and chemokinesis assays for evaluation of cells migration in response to GFs gradient. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MELO, BRUNA A. G.; JODAT, YASAMIN A.; MEHROTRA, SHREYA; CALABRESE, MICHELLE A.; KAMPERMAN, TOM; MANDAL, BIMAN B.; SANTANA, MARIA H. A.; ALSBERG, EBEN; LEIJTEN, JEROEN; SHIN, SU RYON. 3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties. ADVANCED FUNCTIONAL MATERIALS, OCT 2019. (17/02913-4)

MEHROTRA, SHREYA; DE MELO, BRUNA A. G.; HIRANO, MINORU; KEUNG, WENDY; LI, RONALD A.; MANDAL, BIMAN B.; SHIN, SU RYON. Nonmulberry Silk Based Ink for Fabricating Mechanically Robust Cardiac Patches and Endothelialized Myocardium-on-a-Chip Application. ADVANCED FUNCTIONAL MATERIALS, v. 30, n. 12, JAN 2020. (17/02913-4)

DE MELO, BRUNA A. G.; JODAT, YASAMIN A.; MEHROTRA, SHREYA; CALABRESE, MICHELLE A.; KAMPERMAN, TOM; MANDAL, BIMAN B.; SANTANA, MARIA H. A.; ALSBERG, EBEN; LEIJTEN, JEROEN; SHIN, SU RYON. 3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties. ADVANCED FUNCTIONAL MATERIALS, v. 29, n. 51, p. 13-pg., 2019-10-21. (17/02913-4)