Bromelain encapsulation: stability, release profile, and cytotoxicity

• Grant number: 17/05275-9
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: July 14, 2017
• End date: November 13, 2017
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Priscila Gava Mazzola
• Grantee: Janaína Artem Ataide
• Supervisor: Eliana Maria Barbosa Souto
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: Universidade de Coimbra (UC), Portugal
• Associated to the scholarship: 15/15068-5 - DEVELOPMENT OF POLYMERIC PARTICLES AS CARRIER SYSTEMS FOR BIOACTIVE PRODUCTS, BP.MS

Abstract

Bromelain is a collective name for proteolytic enzymes found in species of the Bromeliaceae family, mainly on the stem, fruit and leaves of pineapple (Ananas comosus). The use of this enzyme is highlighted in the food, cosmetic and mainly pharmaceutical industries due to its proteolytic activity. As an enzyme, bromelain use has limitations, since it is easily denatured and degraded, in addition to being unstable in some formulations. A possible alternative to better explore bromelain potential is its encapsulation in nanoparticles. These systems are capable of providing greater stability, efficacy and safety for the encapsulated active and have attracted the interest of the pharmaceutical industry. In this scenatio, chitosan emerges as a promising polymer, mainly due to the biocompatible, biodegradable and mucoadhesive characteristics. This project aims to characterize chitosan-bromelain nanoparticles, as well as to evaluate their release profile and their cytotoxicity in cell lines, with the ultimate goal of making bromelain use viable in formulations.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCO, JULIA GOMES; CEFALI, LETICIA CARAMORI; ATAIDE, JANAINA ARTEM; SANTINI, ANTONELLO; SOUTO, ELIANA B.; MAZZOLA, PRISCILA GAVA. Effect of nanoencapsulation of blueberry (Vaccinium myrtillus): A green source of flavonoids with antioxidant and photoprotective properties. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 23, OCT 2021. (15/25533-7, 17/14757-7, 17/05275-9)

ATAIDE, JANAINA A.; ZANCHETTA, BEATRIZ; SANTOS, ERICA M.; FAVA, ANA LAURA M.; ALVES, THAIS F. R.; CEFALI, LETICIA C.; CHAUD, MARCO, V; OLIVEIRA-NASCIMENTO, LAURA; SOUTO, ELIANA B.; MAZZOLA, PRISCILA G.. Nanotechnology-Based Dressings for Wound Management. PHARMACEUTICALS, v. 15, n. 10, p. 16-pg., 2022-10-01. (15/15068-5, 17/05275-9, 18/07707-6, 16/03444-5, 20/11333-4)

ATAIDE, JANAINA ARTEM; GERIOS, ELOAH FAVERO; MAZZOLA, PRISCILA GAVA; SOUTO, ELIANA B.. Bromelain-loaded nanoparticles: A comprehensive review of the state of the art. ADVANCES IN COLLOID AND INTERFACE SCIENCE, v. 254, p. 48-55, APR 2018. (17/05333-9, 16/03444-5, 17/05275-9)

ATAIDE, JANAINA ARTEM; GERIOS, ELOAH FAVERO; CEFALI, LETICIA CARAMORI; FERNANDES, ANA RITA; TEIXEIRA, MARIA DO CEU; FERREIRA, NUNO R.; TAMBOURGI, ELIAS BASILE; JOZALA, ANGELA FAUSTINO; CHAUD, MARCO VINICIUS; OLIVEIRA-NASCIMENTO, LAURA; et al. Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain-Chitosan Nanoparticles. POLYMERS, v. 11, n. 10, OCT 2019. (17/05333-9, 16/03444-5, 17/05275-9)