Evaluation of the immobilization of anti-SEA polyclonal antibodies on rGO derived from CVD graphene growth

• Grant number: 18/02382-1
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: April 27, 2018
• End date: August 26, 2018
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Ivana Cesarino
• Grantee: Giovanna da Silva Rocha
• Supervisor: Ashok Mulchandani
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of California, Riverside (UCR), United States
• Associated to the scholarship: 17/07288-0 - Development of an immunosensor based on graphene for the detection of enterotoxin A of Staphylococcus aureus in milk samples, BP.IC

Abstract

Staphylococcus aureus is one of the microorganisms involved with food-borne diseases (FBD), mainly associated to the consumption of manually processed and prepared food. The intoxication related with the presence of S. aureus involves the production of staphylococcal enterotoxins (SEs), more specifically of type A, one of the 20 types produced by this bacterium. Once the methods of SEs' detection demand time, specificity and pre-sample treatments, which can be a barrier to fast and effectiveness diagnostics, the development of electrochemical immunosensors based on carbon materials such as nanotubes and graphene are promising. Due to it, this exchange research project aims to evaluate the immobilization of the antibody anti-SEA in reduced oxide graphene (rGO) obtained from graphene synthesized by CVD method with different substances; then, compare the results with the data obtained during the research made in Brazil to develop the most effectiveness, sensitive and accurate immunosensor ever built to detect this type of enterotoxin. (AU)