Clinical investigation of epigenetic modulations in dental pulp tissues with diagnosis of irreversible pulpitis

• Grant number: 19/10755-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 31, 2019
• End date: March 20, 2020
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Brenda Paula Figueiredo de Almeida Gomes
• Grantee: Rodrigo Arruda Vasconcelos
• Supervisor: Phillip Leo Tomson
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: University of Birmingham, England
• Associated to the scholarship: 17/25242-8 - Clinical, microbiological and immunological monitoring of patients undertaken to endodontic therapy with diagnosis of irreversible Pulpitis, BP.DR

Abstract

Bacteria and their products play important roles in the induction of pulpal disease. Biofilms from caries are the main etiological factor in pulpal inflammation. In cases of irreversible pulpitis, the pulp tissue presents accumulation of inflammatory mediators, influencing both destructive and reparative processes. Furthermore epigenetic control regulates the renewal and pluripotency of stem cells such as those present within the dental pulp. Significant epigenetic modifications include DNA methylation, histone acetylation, micro RNA (miRNA) and long non-coding RNA (LncRNA). The aims of this research project are several fold and include: a) characterisation of the epigenetics profile of dental pulps of teeth with irreversible pulpitis; b) comparison of the epigenetic modifications in inflamed dental pulps with normal dental pulps derived from non-carious-teeth; and c) investigation of miRNAs, LncRNAs, DNA methylation and histone acetylation genes/processes and their regulation of inflammation and mineralisation processes. Twenty dental pulps with irreversible pulpitis and 10 dental pulp of teeth with normal pulp will initially be selected for use in this study. Reverse transcriptionquantitative polymerase chain reaction (RTqPCR) using SYBR-green uorescence quantication system in in-house PCR systems will be performed. The results obtained from the tests will be statistically analysed using SPSS for WINDOWS, version 19.0 (SPSS Inc, Chicago, IL, USA). Significance levels will be set at 5% (p<0.05).

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARRUDA-VASCONCELOS, RODRIGO; BARBOSA-RIBEIRO, MARLOS; LOUZADA, LIDIANE M.; LEMOS, BEATRIZ I. N.; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO C. R.; ALMEIDA, JOSE F. A.; MARCIANO, MARINA A.; GOMES, BRENDA P. F. A.. fficacy of 6% Sodium Hypochlorite on Infectious Content of Teeth with Symptomatic Irreversible Pulpiti. JOURNAL OF ENDODONTICS, v. 48, n. 2, p. 179-189, FEB 2022. (17/25242-8, 15/23479-5, 19/10755-5, 19/19300-0)

BARBOSA-RIBEIRO, MARLOS; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; DOS SANTOS, DANIELLE G.; ANDREOTE, FERNANDO D.; GOMES, BRENDA P. F. A.. Microbiological analysis of endodontically treated teeth with apical periodontitis before and after endodontic retreatment. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 4, AUG 2020. (17/25242-8, 15/23479-5, 19/10755-5, 19/19300-0)

BARBOSA-RIBEIRO, MARLOS; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; LIMA, AUGUSTO R.; MARCIANO, MARINA A.; ALMEIDA, JOSE FLAVIO A.; DE-JESUS-SOARES, ADRIANA; ZAIA, ALEXANDRE A.; FERRAZ, CAIO CEZAR R.; GOMES, BRENDA P. F. A.. Microbiological Investigation in Teeth with Persistent/Secondary Endodontic Infection in Different Stages of Root Canal Retreatment. EUROPEAN ENDODONTIC JOURNAL, v. 5, n. 3, p. 219-225, 2020. (17/25242-8, 19/19300-0, 19/10755-5, 12/23697-4, 15/23479-5)

ARRUDA-VASCONCELOS, R.; LOUZADA, L. M.; FERES, M.; TOMSON, P. L.; COOPER, P. R.; GOMES, B. P. F. A.. Investigation of microbial profile, levels of endotoxin and lipoteichoic acid in teeth with symptomatic irreversible pulpitis: a clinical study. International Endodontic Journal, v. 54, n. 1, p. 46-60, JAN 2021. (15/23479-5, 19/10755-5, 17/25242-8, 19/19300-0)

ARRUDA-VASCONCELOS, R.; CHIARELLI-NETO, V. M.; LOUZADA, L. M.; AVEIRO, E.; ALVES-SILVA, E. G.; DE-JESUS-SOARES, A.; FERRAZ, C. C. R.; ALMEIDA, J. F. A.; MARCIANO, M. A.; PECORARI, V. G. A.; et al. Quantitative analysis of culturable bacteria, levels of endotoxins, inflammatory mediators and substance P in teeth with symptomatic irreversible pulpitis and in teeth with vital normal pulp tissues. International Endodontic Journal, v. 56, n. 7, p. 10-pg., 2023-05-02. (21/13871-6, 15/23479-5, 19/10755-5, 17/25242-8, 19/19300-0)

MARTINHO, FREDERICO C.; LEITE, FABIO R. M.; ARRUDA-VASCONCELOS, RODRIGO; DARVEAU, RICHARD P.; GOMES, BRENDA P. F. A.. Influence of Bacterial Profiles in Cytokine and Clinical Features of Endodontic Disease. JOURNAL OF ENDODONTICS, v. 47, n. 8, p. 1265-1271, AUG 2021. (19/10755-5, 15/23479-5, 17/25242-8)

ALVES-SILVA, ESDRAS GABRIEL; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE MENDES; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO CEZAR RANDI; ALMEIDA, JOSE FLAVIO AFFONSO; MARCIANO, MARINA ANGELICA; STEINER-OLIVEIRA, CAROLINA; BELLO-SILVA, MARINA STELLA; SHEMESH, HAGAY; et al. The effect of photodynamic therapy on postoperative pain in teeth with primary endodontic infection. Photodiagnosis and Photodynamic Therapy, v. 37, MAR 2022. (19/19300-0, 17/25242-8, 19/10755-5, 15/23479-5, 19/09115-1)

GOMES, BRENDA P. F. A.; BERBER, VANESSA B.; MARINHO, ARIANE C. S.; LOUZADA, LIDIANE M.; ARRUDA-VASCONCELOS, RODRIGO; PASSINI, MAICON R. Z.; LOPES, ERICA M.; PECORARI, VANESSA G. A.; CHEN, TSUTE; PASTER, BRUCE J.. Chemomechanical preparation influences the microbial community and the levels of LPS, LTA and cytokines in combined endodontic-periodontal lesions: A clinical study. JOURNAL OF PERIODONTAL RESEARCH, DEC 2021. (17/25242-8, 14/01655-3, 19/10755-5, 15/23479-5, 19/19300-0)