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Clinical investigation of epigenetic modulations in dental pulp tissues with diagnosis of irreversible pulpitis

Grant number: 19/10755-5
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 31, 2019
Effective date (End): October 30, 2020
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Brenda Paula Figueiredo de Almeida Gomes
Grantee:Rodrigo Arruda Vasconcelos
Supervisor abroad: Phillip Leo Tomson
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Local de pesquisa : University of Birmingham, England  
Associated to the scholarship:17/25242-8 - Clinical, microbiological and immunological monitoring of patients undertaken to endodontic therapy with diagnosis of irreversible Pulpitis, BP.DR

Abstract

Bacteria and their products play important roles in the induction of pulpal disease. Biofilms from caries are the main etiological factor in pulpal inflammation. In cases of irreversible pulpitis, the pulp tissue presents accumulation of inflammatory mediators, influencing both destructive and reparative processes. Furthermore epigenetic control regulates the renewal and pluripotency of stem cells such as those present within the dental pulp. Significant epigenetic modifications include DNA methylation, histone acetylation, micro RNA (miRNA) and long non-coding RNA (LncRNA). The aims of this research project are several fold and include: a) characterisation of the epigenetics profile of dental pulps of teeth with irreversible pulpitis; b) comparison of the epigenetic modifications in inflamed dental pulps with normal dental pulps derived from non-carious-teeth; and c) investigation of miRNAs, LncRNAs, DNA methylation and histone acetylation genes/processes and their regulation of inflammation and mineralisation processes. Twenty dental pulps with irreversible pulpitis and 10 dental pulp of teeth with normal pulp will initially be selected for use in this study. Reverse transcriptionquantitative polymerase chain reaction (RTqPCR) using SYBR-green uorescence quantication system in in-house PCR systems will be performed. The results obtained from the tests will be statistically analysed using SPSS for WINDOWS, version 19.0 (SPSS Inc, Chicago, IL, USA). Significance levels will be set at 5% (p<0.05).

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