Evaluation of acute toxicity and gastroprotection activity of chrysin flavone in female rodents

• Grant number: 19/12510-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2019
• End date: October 31, 2020
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Raquel de Cássia dos Santos
• Grantee: Quélita Cristina Pereira
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil

Abstract

The present project aims to evaluate the pharmacological potential of flavone chrysin, a flavonoid naturally found in honey, propolis and various plant species, such as passion fruit (Passiflora coerulea) against gastric ulcer induced by ethanol and to evaluate if chrysin presents toxicological changes in female mice. Chrysin shows promising gastroprotective activity in male mice (FAPESP process 2016/ 21102-4). In order to investigate their safety, an acute toxicity test will be performed, as indicated by the OECD, in which female mice will be treated with single dose of chrysin (2000 mg/kg), having several parameters compared to the vehicle group (histological analysis of the heart, kidneys and liver, analysis of plasma levels of AST, gamma-GT, AST, urea, creatinine, glucose). Additionally, based on the differences between males and females, the absolute ethanol induction model will be performed in females, to compare the activity in the same doses already evaluated in males. Different studies indicate that the pharmacological response differs in males and females, thus, this work aims to analyze if the chrysin promotes gastroprotection also in females, if this response resembles that found in males and what mechanisms are involved, analyzing macroscopic parameters of gastric lesion, antioxidants (SOD, CAT, GSH), inflammatory (MPO) and morphological (histology). The results obtained will provide data on the safety and toxicity of flavone chrysin as well as whether the activity against gastric ulcer is similar to that found in male mice, as well as provide scientific subsidies for the future incorporation of this compound as a therapeutic option. (AU)