Development of novel immunosensor architechtures for the detection of Huntington and Parkinson Diseases biomarkers

• Grant number: 19/23342-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2020
• End date: July 31, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Bruno Campos Janegitz
• Grantee: Luiz Otávio Orzari
• Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil
• Associated research grant: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use, AP.BTA.TEM
• Associated scholarship(s): 22/13157-4 - Screen-printed electrodes modified with AuBi nanoparticles for direct oxidation of alcohols and glucose in alkaline direct fuel cells, BE.EP.DD

Abstract

Nowadays, Huntington's and Parkinson's Diseases are two of the main neurodegenerative diseases that plague mankind. Having their first symptoms after adulthood, they lead to a devastating degradation of the motor and nervous systems, incurable. Thus, early diagnosis and control are essential for a better quality of life of patients, so that the study of biomarkers for both is considerably growing. In search of a relatively low cost alternative, low waste generation and fast response, this project proposes the construction of new electrochemical devices for the biosensing of these biomarkers. Both proposals aim the formation of labelfree immunosensors with polyclonal antibodies, which will be produced from two research fronts: the first being of carbon-conductive inks; and the second, with three-dimensional printing of electrode systems using carbon conductive filaments. With metallic nanoparticles, it will be possible to modify both the surface of the electrodes or directly in the formulation of these materials, pursuing a greater interaction between device and antibody/antigen molecules. In addition, during the application of these architectures, studies about the electrochemical profiles with various probes of different properties will be carried out, seeking a better clarification of each system interactions. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FREITAS, RAFAELA C.; ORZARI, LUIZ O.; FERREIRA, LUIS M. C.; PAIXAO, THIAGO R. L. C.; COLTRO, WENDELL K. T.; VICENTINI, FERNANDO C.; JANEGITZ, BRUNO C.. Electrochemical determination of melatonin using disposable self-adhesive inked paper electrode. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 897, SEP 15 2021. (17/21097-3, 19/24428-6, 19/23342-0, 18/08782-1)

DANIELE, GIOVANNI G.; DE SOUZA, DANIEL C.; DE OLIVEIRA, PAULO ROBERTO; ORZARI, LUIZ O.; BLASQUES, RODRIGO V.; GERMSCHEIDT, RAFAEL L.; DA SILVA, EMILLY C.; POCRIFKA, LEANDRO A.; BONACIN, JULIANO A.; JANEGITZ, BRUNO C.. Development of Disposable and Flexible Supercapacitor Based on Carbonaceous and Ecofriendly Materials. C-JOURNAL OF CARBON RESEARCH, v. 8, n. 2, p. 14-pg., 2022-06-01. (17/23960-0, 17/11986-5, 19/23342-0)

CAMARGO, JESSICA ROCHA; ORZARI, LUIZ OTAVIO; ARAUJO, DIELE APARECIDA GOUVEIA; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; ROCHA, DIEGO PESSOA; DOS SANTOS, ANDRE LUIZ; TAKEUCHI, REGINA MASSAKO; MUNOZ, RODRIGO ALEJANDRO ABARZA; BONACIN, JULIANO ALVES; et al. Development of conductive inks for electrochemical sensors and biosensors. Microchemical Journal, v. 164, MAY 2021. (17/21097-3, 19/23342-0, 13/22127-2, 19/23177-0, 19/00473-2, 17/23960-0, 19/01844-4)

ORZARI, LUIZ OTAVIO; ASSUMPC, MONICA HELENA MARCON TEIXEIRA; NANDENHA, JULIO; NETO, ALMIR OLIVEIRA; JUNIOR, LUIZ HUMBERTO MARCOLINO; BERGAMINI, MARCIO; JANEGITZ, BRUNO CAMPOS. Pd, Ag and Bi carbon-supported electrocatalysts as electrochemical multifunctional materials for ethanol oxidation and dopamine determination. Electrochimica Acta, v. 428, p. 13-pg., 2022-08-03. (17/21097-3, 19/23342-0)

DE FREITAS, RAFAELA CRISTINA; ORZARI, LUIZ OTAVIO; DE OLIVEIRA, PAULO ROBERTO; JANEGITZ, BRUNO CAMPOS. Pd and Ag Binary Nanoparticles Supported on Carbon Black and Tapioca for Nitrite Electrochemical Detection. Journal of the Electrochemical Society, v. 168, n. 11, NOV 1 2021. (19/01844-4, 19/23342-0, 19/24428-6, 17/21097-3)

GUIMARAES, VITORIA PISTORI; NANDENHA, JULIO; ORZARI, LUIZ OTAVIO; FATIBELLO-FILHO, ORLANDO; OLIVEIRA NETO, ALMIR; JANEGITZ, BRUNO CAMPOS; VICENTINI, FERNANDO CAMPANHA; MARCON TEIXEIRA ASSUMPCAO, MONICA HELENA. Effect of TiO2 and Synthesis Strategies on Formate Oxidation: Electrochemical and Fuel Cell Approaches. ELECTROCATALYSIS, v. 14, n. 2, p. 11-pg., 2022-11-03. (17/21097-3, 20/01050-5, 19/23342-0)