Comparison of mechanisms of bone loss in patients undergoing Roux-en-Y gastric bypass or sleeve gastrectomy.

• Grant number: 19/24782-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2020
• End date: August 31, 2023
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Bruno Gualano
• Grantee: Igor Hisashi Murai
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 16/00006-7 - Evaluation of Bone Metabolism Parameters by HR-pQCT and Histomorphometry in Rheumatoid Arthritis and Juvenile Idiopathic Arthritis Patients: Association with Clinical and Genetic Variables, AP.TEM

Abstract

The research project elaborated in this proposal follows the candidate's doctorate research line (FAPESP 15/02833-5 and 16/10993-5), which aimed to evaluate the effects of exercise training on bone metabolism in women with severe obesity after bariatric surgery. Bariatric surgery is the treatment of choice for severe obesity, as this is the only intervention capable of inducing a significant weight loss in this condition, resulting in an improvement and/or complete remission of the obesity-related comorbidities. However, bariatric surgery-induced bone loss has been consistently reported in previous studies, which may increase the fracture risk. Despite a growing body of evidence showing bone loss after bariatric surgery in recent years, little is known about the mechanisms that cause this biological phenomenon in different surgical procedures. Therefore, this project aims to compare the mechanisms of bone loss in women undergoing the most common bariatric surgery procedures, which are the Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG). In order to test our hypothesis, 30 women with severe obesity, 15 of them eligible for RYGB and 15 for SG, will be submitted to bone metabolism assessments, including iliac crest bone biopsy in order to evaluate the cellular and molecular mechanisms of bone tissue deterioration; bone densitometry; high-resolution peripheral quantitative computed tomography; blood samples to evaluate bone turnover markers and; body composition, before and 12 months after surgery. A mixed model analysis for repeated measures will be performed to assess the effects of surgical procedures on bone parameters along the time, and Tukey post hoc will be employed for multiple comparisons. The significance level for testing null hypothesis will be 5%.

Scientific publications

(11)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

MURAI, IGOR H.; FERNANDES, ALAN L.; SALES, LUCAS P.; PINTO, ANA J.; GOESSLER, KARLA F.; DURAN, CAMILA S. C.; SILVA, CARLA B. R.; FRANCO, ANDRE S.; MACEDO, MARINA B.; DALMOLIN, HENRIQUE H. H.; et al. Effect of a Single High Dose of Vitamin D-3 on Hospital Length of Stay in Patients With Moderate to Severe COVID-19 A Randomized Clinical Trial. JAMA, v. 325, n. 11, FEB 2021. (20/11102-2, 15/26937-4, 17/13552-2, 19/24782-4, 19/18039-7, 20/05752-4, 16/00006-7)

FRANCO, ANDRE S.; MURAI, IGOR H.; TAKAYAMA, LILIAM; CAPARBO, VALERIA F.; MARCHI, LUAN L.; VELLOSO, ELVIRA D. R. P.; PEREIRA, ROSA M. R.. Assessment of Bone Microarchitecture in Patients with Systemic Mastocytosis and its Association with Clinical and Biochemical Parameters of the Disease. CALCIFIED TISSUE INTERNATIONAL, v. N/A, p. 10-pg., 2023-06-09. (19/24782-4)

SALES, LUCAS P.; SOUZA, LUCAS V. B.; FERNANDES, ALAN L.; MURAI, IGOR H.; SANTOS, MAYARA D.; VENDRAMINI, MARGARETE B. G.; OLIVEIRA, RICARDO M.; FIGUEIREDO, CAMILLE P.; CAPARBO, VALERIA F.; GUALANO, BRUNO; et al. Effect of vitamin D₃ on antiphospholipid antibodies in hospitalized patients with moderate to severe COVID-19. Clinics, v. 79, p. 7-pg., 2024-08-28. (19/24782-4, 20/11102-2, 20/07098-0, 20/05752-4)

IGOR H. MURAI; ALAN L. FERNANDES; LEILA ANTONANGELO; BRUNO GUALANO; ROSA MARIA RODRIGUES PEREIRA. Effect of a Single High-Dose Vitamin D3 on the Length of Hospital Stay of Severely 25-Hydroxyvitamin D-Deficient Patients with COVID-19. Clinics, v. 76, 2021-11-26. (20/05752-4, 20/11102-2, 19/24782-4)

MURAI, IGOR H.; FERNANDES, ALAN L.; ANTONANGELO, LEILA; GUALANO, BRUNO; RODRIGUES PEREIRA, ROSA MARIA. Effect of a Single High-Dose Vitamin D-3 on the Length of Hospital Stay of Severely 25-Hydroxyvitamin D-Deficient Patients with COVID-19. Clinics, v. 76, 2021. (20/11102-2, 19/24782-4, 20/05752-4)

PINTO, ANA J.; GOESSLER, KARLA F.; FERNANDES, ALAN L.; MURAI, IGOR H.; SALES, LUCAS P.; REIS, BRUNA Z.; SANTOS, MAYARA DINIZ; ROSCHEL, HAMILTON; PEREIRA, ROSA M. R.; GUALANO, BRUNO. No independent associations between physical activity and clinical outcomes among hospitalized patients with moderate to severe COVID-19. JOURNAL OF SPORT AND HEALTH SCIENCE, v. 10, n. 6, p. 690-696, DEC 2021. (16/00006-7, 20/05752-4, 15/26937-4, 19/18039-7, 19/24782-4, 17/13552-2, 20/11102-2)

VALENTE, GABRIEL V.; MACHADO, LUANA G.; FIGUEIREDO, CAMILLE P.; CAPARBO, VALERIA F.; TAKAYAMA, LILIAM; FRANCO, ANDRE S.; FERNANDES, ALAN L.; OLIVEIRA, RICARDO M.; PEREIRA, ROSA M. R.; DOMICIANO, DIOGO S.. Visceral adipose tissue measured by DXA predicts metabolic syndrome in low-income community-dwelling elderly: Insights from the Sao Paulo Aging & Health (SPAH) study. BONE, v. 190, p. 8-pg., 2025-01-01. (19/24782-4)

FERNANDES, ALAN L. L.; SALES, LUCAS P. P.; SANTOS, MAYARA D. D.; CAPARBO, VALERIA F. F.; MURAI, IGOR H. H.; PEREIRA, ROSA M. R.. Persistent or new symptoms 1 year after a single high dose of vitamin D-3 in patients with moderate to severe COVID-19. FRONTIERS IN NUTRITION, v. 9, p. 10-pg., 2022-09-13. (20/11102-2, 19/24782-4, 20/07098-0, 20/05752-4)

REIS, BRUNA Z.; FERNANDES, ALAN L.; SALES, LUCAS P.; SANTOS, MAYARA D.; DOS SANTOS, CAROLINE C.; PINTO, ANA J.; GOESSLER, KARLA F.; FRANCO, ANDRE S.; DURAN, CAMILA S. C.; SILVA, CARLA B. R.; et al. Influence of vitamin D status on hospital length of stay and prognosis in hospitalized patients with moderate to severe COVID-19: a multicenter prospective cohort study. AMERICAN JOURNAL OF CLINICAL NUTRITION, v. 114, n. 2, p. 598-604, AUG 2021. (17/13552-2, 15/26937-4, 19/18039-7, 16/00006-7, 20/05752-4, 19/24782-4, 20/11102-2)

MURAI, IGOR H.; FERNANDES, ALAN L.; SALES, LUCAS P.; PINTO, ANA J.; GOESSLER, KARLA F.; DURAN, CAMILA S. C.; SILVA, CARLA B. R.; FRANCO, ANDRE S.; MACEDO, MARINA B.; DALMOLIN, HENRIQUE H. H.; et al. Effect of a Single High Dose of Vitamin D-3 on Hospital Length of Stay in Patients With Moderate to Severe COVID-19 A Randomized Clinical Trial. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, v. 325, n. 11, p. 8-pg., 2021-02-17. (20/11102-2, 16/00006-7, 19/24782-4, 15/26937-4, 19/18039-7, 20/05752-4, 17/13552-2)

FERNANDES, ALAN L.; MURAI, IGOR H.; REIS, BRUNA Z.; SALES, LUCAS P.; SANTOS, MAYARA D.; PINTO, ANA J.; GOESSLER, KARLA F.; DURAN, CAMILA S. C.; SILVA, CARLA B. R.; FRANCO, ANDRE S.; et al. Effect of a single high dose of vitamin D-3 on cytokines, chemokines, and growth factor in patients with moderate to severe COVID-19. AMERICAN JOURNAL OF CLINICAL NUTRITION, v. 115, n. 3, p. 9-pg., 2022-03-04. (20/11102-2, 19/18039-7, 19/24782-4, 20/05752-4)