Obtention of improved electrochemical sensors by modifying 3D printed electrodes with biofilms obtained from beeswax and carbon nanomaterials for the detection of harmful compounds in honey

• Grant number: 22/06145-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2022
• End date: June 30, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Bruno Campos Janegitz
• Grantee: Jéssica Santos Stefano
• Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil
• Associated research grant: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use, AP.BTA.TEM

Abstract

The use of pesticides has been increasingly used in crop cultivation. Despite their effectiveness, these compounds are highly harmful to human and animal health, and have contaminated living beings that have direct contact with plants, such as bees, causing damage to health or even leading to death. In addition to direct contamination, the consumption of products from bees, such as honey, can be a source of human contamination by pesticides, and the determination of these compounds in honey can be a way of controlling the use of pesticides. In this aspect, reliable, fast and low-cost analysis techniques are of great interest, especially electrochemical techniques. The use of 3D printing technology has been increasingly explored to obtain versatile and low-cost electrochemical sensors in a simple way, in the laboratory. The use of these sensors can be a great differential due to the ease of obtaining, potential for portability of systems or even creation of wearable sensors due to their versatility in design and material used. Obtaining chemically modified electrodes becomes very attractive in obtaining improved sensors, especially when it involves the use of environmentally friendly materials in the formation of carbon-based thin films. Therefore, in this project, we intend to use beeswax as a base material for electrode modification, obtaining improved sensors used in the detection of contaminants in honey samples. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, LUIZ R. G.; STEFANO, JESSICA S.; ORZARI, LUIZ O.; BRAZACA, LAIS C.; CARRILHO, EMANUEL; MARCOLINO-JUNIOR, LUIZ H.; BERGAMINI, MARCIO F.; MUNOZ, RODRIGO A. A.; JANEGITZ, BRUNO C.. Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes. BIOSENSORS-BASEL, v. 12, n. 8, p. 20-pg., 2022-08-01. (17/21097-3, 22/06145-0, 18/19750-3)

FABRI, JOCIELI; SILVA, LUIZ R. G.; STEFANO, JESSICA S.; PEREIRA, JIAN F. S.; COCCO, DANIEL R.; MUNOZ, RODRIGO A. A.; ROCHA, DIEGO P.. In situ electrochemical determination of resorcinol using a fully 3D printed apparatus. Microchemical Journal, v. 191, p. 8-pg., 2023-05-08. (17/21097-3, 22/06145-0)

SILVA, LUIZ R. G.; STEFANO, JESSICA S.; CRAPNELL, ROBERT D.; BANKS, CRAIG E.; JANEGITZ, BRUNO C.. Additive manufactured microfluidic device for electrochemical detection of carbendazim in honey samples. TALANTA OPEN, v. 7, p. 8-pg., 2023-04-05. (17/21097-3, 22/06145-0)

STEFANO, JESSICA SANTOS; GUTERRES E SILVA, LUIZ RICARDO; JANEGITZ, BRUNO CAMPOS. New carbon black-based conductive filaments for the additive manufacture of improved electrochemical sensors by fused deposition modeling. Microchimica Acta, v. 189, n. 11, p. 16-pg., 2022-11-01. (22/06145-0, 17/21097-3)

CARVALHO, JEFFERSON H. S.; STEFANO, JESSICA S.; BRAZACA, LAIS C.; JANEGITZ, BRUNO C.. New conductive ink based on carbon nanotubes and glass varnish for the construction of a disposable electrochemical sensor. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 937, p. 13-pg., 2023-04-07. (17/21097-3, 22/06145-0, 20/11336-3)

STEFANO, JESSICA SANTOS; SILVA, LUIZ RICARDO GUTERRES E; KALINKE, CRISTIANE; DE OLIVEIRA, PAULO ROBERTO; CRAPNELL, ROBERT D.; BRAZACA, LAIS CANNIATTI; BONACIN, JULIANO ALVES; CAMPUZANO, SUSANA; BANKS, CRAIG E.; JANEGITZ, BRUNO CAMPOS. Human monkeypox virus: Detection methods and perspectives for diagnostics. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 167, p. 18-pg., 2023-10-01. (17/21097-3, 21/07989-4, 22/06145-0, 13/22127-2)

DA SILVA, VINICIUS A. O. P.; STEFANO, JESSICA S.; KALINKE, CRISTIANE; BONACIN, JULIANO. A. A.; JANEGITZ, BRUNO. C. C.. Additive Manufacturing Sensor for Stress Biomarker Detection. CHEMOSENSORS, v. 11, n. 5, p. 14-pg., 2023-05-20. (22/06145-0, 19/00473-2, 17/21097-3, 21/03179-8, 21/07989-4)

SILVA, LUIZ RICARDO GUTERRES; STEFANO, JESSICA SANTOS; NOCELLI, ROBERTA CORNELIO FERREIRA; JANEGITZ, BRUNO CAMPOS. 3D electrochemical device obtained by additive manufacturing for sequential determination of paraquat and carbendazim in food samples. Food Chemistry, v. 406, p. 9-pg., 2023-04-16. (17/21097-3, 22/06145-0, 18/19750-3)

SILVA, LUIZ R. G.; CARVALHO, JEFFERSON H. S.; STEFANO, JESSICA S.; OLIVEIRA, GEISER G.; PRAKASH, JAI; JANEGITZ, BRUNO C.. Electrochemical sensors and biosensors based on nanodiamonds: A review. MATERIALS TODAY COMMUNICATIONS, v. 35, p. 14-pg., 2023-05-18. (17/21097-3, 22/06145-0, 20/11336-3)

SILVA, LUIZ RICARDO GUTERRES; STEFANO, JESSICA SANTOS; CRAPNELL, ROBERT D.; BANKS, CRAIG E.; JANEGITZ, BRUNO CAMPOS. Additive manufacturing of carbon black immunosensors based on covalent immobilization for portable electrochemical detection of SARS-CoV-2 spike S1 protein. TALANTA OPEN, v. 8, p. 9-pg., 2023-12-01. (17/21097-3, 22/06145-0)