Interaction of mirsinoic acid with membrane models using Langmuir monolayers

• Grant number: 23/07185-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2023
• End date: August 31, 2025
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Ana Gabrieli Araujo dos Santos
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM

Abstract

In this project, we intend to study the action of mirsinoic acid, a compound with potential antimicrobial and antitumor activity, with Langmuir films of phospholipids, which will serve as cell membrane models. The phospholipids to be used will be dipalmitoylphosphatidyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidyl serine (DPPS), which will be co-spread with the bioactive. The interaction of the bioactive with the lipid monolayers will be evaluated through measurements of pressure isotherms and surface potential, Brewster angle microscopy, infrared spectroscopy, and surface rheology. The results will be evaluated from the perspective of possible biological implications, inferred from the effects caused by the bioactive on artificial membranes.