| Grant number: | 24/03926-6 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | December 01, 2024 |
| End date: | November 30, 2026 |
| Field of knowledge: | Agronomical Sciences - Food Science and Technology - Food Science |
| Principal Investigator: | Neuza Mariko Aymoto Hassimotto |
| Grantee: | Layanne Nascimento Fraga |
| Host Institution: | Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 13/07914-8 - FoRC - Food Research Center, AP.CEPID |
| Associated scholarship(s): | 25/21815-0 - Effects of Proanthocyanidin Intake from "Maracujá -alho" (Passiflora tenuifila Killip): A Metabolomic Approach, BE.EP.PD |
Abstract The fruit of Passiflora tenuifila Killip, a native passion fruit from Brazilian Cerrado, is rich in bioactive compounds associated with reducing the occurrence of cardiometabolic diseases. Our research group demonstrated that P. tenuifila fruits are rich in phenolic compounds, mainly proanthocyanidins, and their intake promotes the improvement of some cardiometabolic biomarkers. The biological action has been related with their gut-derived catabolites, instead of their native structure, including phenyl-y-varelolactones and valeric acid derivates. The type and amount of these catabolites depends on the chemical structure and gut microbiota composition. Thus, considering that the biological response depends on their bioactive gut-derived metabolites and gut-derived catabolites formed, this work aims to evaluate the bioavailability of the phenolic compounds from P. tenuifila and their influence on gut microbiota; besides, evaluating the potential molecular target of the biological action. Furthermore, a metabolomic approach will be used to study the effect of ingestion on the human metabolome. For this, the samples that will be analyzed were obtained from a randomized crossover clinical trial, conducted between March and June/2023. Volunteers ingested lyophilized P. tenuifila or a placebo for 15 consecutive days. Blood, feces, and urine samples were collected at time zero and after 15 days. Metabolization/excretion of phenolic metabolites will be evaluated in 24-hour urine collected in intervals of 0-4h, 4-8h, 8-12h, and 12-24h, after the first dose of P. tenuifila (beginning of the intervention) and at the end of the trial by LC-qToF-MS/MS and triple quadrupole LC-MS/MS. The intestinal microbiome profile will be analyzed in feces by 16S. Subsequently, the AhR binding potential of the main catabolites will be evaluated by molecular docking and in cell culture. Thus, it is expected to identify the main gut-derived catabolites of proanthocyanidins with potential biological action, evaluating the bidirectional interactions between gut microbiota and phenolic metabolism, and also potential molecular targets of the action of these compounds. In addition, the study of native species can enhance the socioeconomic development of the region, as well as enhance and stimulate Brazilian biodiversity. | |
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