Evaluation of some microorganism from endogenous intestinal microbiota of normal weight, overweight and obese schoolchildren.

Author(s)

Aline Ignacio Silvestre da Silva

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-04-16

Examining board members

Viviane Nakano; Waldir Pereira Elias Junior; Luiz Vicente Ribeiro Ferreira da Silva Filho

• Advisor: Viviane Nakano

Abstract

The aim of this study was to evaluate some microorganism from endogenous intestinal microbiota of normal weight (30), overweight (24) and obese (30) children between 3 and 11 years, from fecal samples. It was performed the isolation of species of Bacteroides, Parabacteroides and Clostridium; the identification of B. fragilis and C. perfringens enterotoxigenic; and the quantitative detection by PCR (SybrGreen) B. fragilis, B. vulgatus, P. distasonis, C. perfringens, C. difficile, Bifidobacterium spp., Lactobacillus spp., Bacteroidales and Clostridium (cluster I). The species C. perfringens and B. vulgatus were the most isolated; no isolated B. fragilis was enterotoxigenic; all C. perfringens were classified as type A and these 8.7% and 12.2% harbored tpeL and netB genes, respectively. C. perfringens, C. difficile and Bifidobacterium spp. were in greater quantity in normal weight children while obese and overweight showed a higher number of Lactobacillus spp. and Bacteroidales. (AU)

• FAPESP's process: 12/10659-7 - Evaluation of some microorganisms of the endogenous intestinal microbiota of children with normal weight, obese and overweight in school-age
• Grantee: Aline Ignacio Silvestre da Silva
• Support Opportunities: Scholarships in Brazil - Master