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Impact of a fermented soy probiotic beverage supplemented with acerola by-product on the composition and metabolic activity of obese and lean human intestinal microbiota evaluated in vitro

Grant number: 16/07940-7
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): June 24, 2016
Effective date (End): February 23, 2017
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Susana Marta Isay Saad
Grantee:Antônio Diogo Silva Vieira
Supervisor: Koen Venema
Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Maastricht University, Venlo (UM), Netherlands  
Associated to the scholarship:13/19346-4 - Impact of the incorporation of probiotic strains and fruit by-products in a fermented synbiotic soy product on the composition and metabolic activity of the gut microbiota in vitro, BP.DR


The incidence of obesity and its associated disorders has increased in recent years worldwide. Obesity is associated with increased chronic diseases like cardiovascular disease, dyslipidemia, atherosclerosis, hypertension, diabetes, hepatic diseases, and cancer. The gut microbiota has been suggested to be an environmental factor that affects adiposity and obesity. Studies have demonstrated that obese individuals have an altered gut microbiota compared to lean controls. In view of these, the aim of this project is to evaluate the impact of fermented soy probiotic beverages supplemented with acerola by-product on the composition and metabolic activity of human lean and obese microbiota. For this purpose, a validated in vitro model called TIM-2, available at the Maastricht University, campus Venlo, Venlo, the Netherlands will be used, which simulates the normal conditions of the lumen of the proximal colon, with all parameters controlled by a computer. Samples will be collected from TIM-2 for quantification of probiotic microorganisms (Lactobacillus acidophilus LA-5 and Bifidobacterium longum BB-46), Lactobacillus spp., Bifidobacterium spp., Clostridium spp., and total bacteria, using the quantitative PCR method (qPCR) with specific primers and gut microbiota profiling will be using methods Next-Generation Sequencing (NGS) Ilumina Hiseq. Additionally, the concentration of short-chain fatty acids, lactate, and ammonia produced during the assays will be determined through the intestinal tract simulation model transit. These tests will be conducted at the Maastricht University, campus Venlo, Venlo, Netherlands, under supervision of Prof. Dr. Koen Venema. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA VIEIRA, ANTONIO DIOGO; DE SOUZA, CARLOTA BUSSOLO; PADILHA, MARINA; ZOETENDAL, ERWIN GERARD; SMIDT, HAUKE; ISAY SAAD, SUSANA MARTA; VENEMA, KOEN. Impact of a fermented soy beverage supplemented with acerola by-product on the gut microbiota from lean and obese subjects using an in vitro model of the human colon. Applied Microbiology and Biotechnology, v. 105, n. 9, p. 3771-3785, . (13/26435-3, 13/19346-4, 13/50506-8, 16/07940-7)

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