CV Lattes
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Universidade de São Paulo (USP). Instituto de Biociências (IB) (Institutional affiliation for the last research proposal) Birthplace: Brazil
Bachelor and Licenced in Biological Sciences by the Institute of Biosciences USP, with Doctorate in Biochemistry by the Institute of Chemistry USP. Currently is a Full Professor of the Department of Physiology at IBUSP. Developed research projects with organisms of the family Trypanosomatidae, mainly Leishmania and Trypanosoma. Described DNA probes for identifying these organisms using various molecular targets and applying techniques such as hybridization and PCR. In basic molecular biology, studied the organization of RNA polymerase I promoters and the regulation of the expression of ribosomal RNA genes. Also studied the metabolism of Leishmania to understand the relation of the parasite with the host cell, with the objective of describing chemotherapeutic targets. Worked in the area of ​​higher education, having acted in the coordination of the Molecular Sciences Course, (PRG-USP), a course dedicated to training scientists (2003 to 2008). Is a member of the Academy of Sciences of the State of São Paulo. A former member of the Board of Directors of the Brazilian Society of Protozoology (SBPz). Former member of the Board of the Brazilian Society for the Advancement of Science (SBPC) and is currently a member of the Board of Directors of tSBPC. Also represented the SBPC at the National Animal Experimentation Control Council (CONCEA). President of `Brazilian Society of Protozoology. (Source: Lattes Curriculum)
Leishmania is the protozoan responsible for leishmaniasis, a neglected disease that in Brazil causes about 100,000 new cases per year. In its life cycle, the parasite is found in the midgut of an insect or inside the phagolysosome in the macrophage of the mammalian host. The different stages of parasite life cycle influence the regulation of gene expression, which occurs at post-transcrip…
The biology of parasites belonging to Leishmania genus offers unique features as model for studying the host - pathogen interaction. Here we propose three sub-projects to study biochemical and physiological aspects of this interaction, primarily upon infe (AU)
The biology of parasites belonging to Leishmania genus offers unique features as model for studying the host - pathogen interaction. Here we propose three sub-projects to study biochemical and physiological aspects of this interaction, primarily upon infection of macrophages in the mammalian host. The objectives of the project are: To correlate the sub-cellular localization of arginase in…
The addition of a capped mini-exon by trans-splicing is an essential step in the maturation of RNA polymerase II transcribed polycistronic pre-mRNAs in trypanosomatids. The process is coupled to polyadenylation of the previous messenger in the precursor RNA molecule. Previous observation of expression of reporter proteins, which transcription was driven by RNA polymerase I (RNA pol I) lea…
(Only some records are available in English at this moment)
To evaluate the effects of sleep fractionation (FS), cytokine production and the profile of miRNAs involved in Th1 and Th2 responses in mice (C57BL / 6) infected by L. amazonensis, mainly miRNA-294, miRNA-721, miRNA -let7e, miRNA-30e and miRNA-302e and their roles in arginine metabolism.
Leishmania is an obligate intracellular parasite in the mammalian host, infecting phagocytic cells of the immune system. Inside the macrophage, the amastigote form is capable of subverting the hostile environment of the phagolysosome modulating host gene expression for its survival. L-arginine is a common substrate for both Nitric Oxide (NO) or polyamines production by macrophage enzymes …
Leishmania is a protozoan parasite that alternates its life cycle between the sand fly and the mammalian hosts. This alternation involves environmental changes and submits the parasite to dynamic modifications in morphology, metabolism, cellular signaling and gene expression regulation to allow a rapid adaptation to new conditions. Our research group have been studying the role of arginas…
Leishmania is a protozoan parasite that causes leishmaniases, a disease that presents clinical manifestations ranging from cutaneous lesions to visceral damage, depending on the parasite species and the host's immunological conditions. Leishmania is auxotrophic for L-arginine and presents the machinery for its uptake and metabolism. The amino acid is the common substrate for the enzymes a…
The establishment of Leishmania infection in macrophages depends on the subversion of the immune response of this host cell to the parasite. One of these subversion mechanisms consists in the modulation of the L-arginine metabolism, a common substrate of nitric oxide (NO) and polyamines production pathways. This regulation directly influences the survival of the amastigote in the phagolys…
These project was based in the hypothesis that the Leishmania (Leishmania) amazonensis could alters the microRNAs (miRNA) profile of infected macrophages and consequently the expression of mRNAs involved in the polyamines and nitric oxide production. Previously results point for modulation of miRNAs/mRNAs involved in the L-arginine metabolism to produce polyamines, essentials for parasite…
(Only some records are available in English at this moment)
| 1 / 1 | Ongoing research grants |
| 14 / 7 | Completed research grants |
| 3 / 3 | Ongoing scholarships in Brazil |
| 30 / 8 | Completed scholarships in Brazil |
| 1 / 0 | Completed scholarships abroad |
| 49 / 19 | All research grants and scholarships |
| Associated processes | |
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
| Publications | 3 |
| Citations | 46 |
| Cit./Article | 15.3 |
| Data from Web of Science | |
DOS SANTOS, MARCOS GONZAGA; PAES, LISVANE SILVA; ZAMPIERI, RICARDO ANDRADE; LARANJEIRA DA SILVA, MARIA FERNANDA; SILBER, ARIEL MARIANO; FLOETER-WINTER, LUCILE MARIA. Biochemical characterization of serine transport in Leishmania (Leishmania) amazonensis. Molecular and Biochemical Parasitology, v. 163, n. 2, p. 107-113, Feb. 2009. Web of Science Citations: 6. (06/00019-0)
LARANJEIRA-SILVA, MARIA FERNANDA; ZAMPIERI, RICARDO A.; MUXEL, SANDRA M.; FLOETER-WINTER, LUCILE MARIA; MARKUS, REGINA P.. Melatonin attenuates Leishmania (L.) amazonensis infection by modulating arginine metabolism. Journal of Pineal Research, v. 59, n. 4, p. 478-487, NOV 2015. Web of Science Citations: 18. (10/52688-8, 13/13691-1)
ORLANDO, TEREZA CRISTINA; MAYER, MÁRIO GUSTAVO; CAMPBELL, DAVID A.; STURM, NANCY R.; FLOETER-WINTER, LUCILE MARIA. RNA polymerase I promoter and splice acceptor site recognition affect gene expression in non-pathogenic Leishmania species. Memórias do Instituto Oswaldo Cruz, v. 102, n. 7, p. 891-894, Nov. 2007. (06/00019-0)
MUXEL, SANDRA MARCIA; LARANJEIRA-SILVA, MARIA FERNANDA; CARVALHO-SOUSA, CLAUDIA EMANUELLE; FLOETER-WINTER, LUCILE MARIA; MARKUS, REGINA P.. The RelA/cRel nuclear factor-B (NF-B) dimer, crucial for inflammation resolution, mediates the transcription of the key enzyme in melatonin synthesis in RAW 264.7 macrophages. Journal of Pineal Research, v. 60, n. 4, p. 394-404, MAY 2016. Web of Science Citations: 22. (10/52688-8, 14/50717-1, 12/15263-4, 13/13691-1)
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Maria Fernanda Laranjeira da. Relação entre a localização celular da enzima arginase de Leishmania (Leishmania) amazonensis e seu papel na infecção de macrófagos murinos. Tese (Doutorado) - Instituto de Biociências. Universidade de São Paulo (USP). São Paulo. (06/50482-8)
MARTINS, Emerson Augusto Castilho. Caracterização fisiológica e genética do transporte de arginina em Leishmania (Leishmania) amazonensis. Tese (Doutorado) - Instituto de Biociências. Universidade de São Paulo (USP). São Paulo. (06/07143-8)
SANTOS, Marcos Gonzaga dos. Papel funcional da fosfatidilserina de Leishmania (Leishmania) amazonensis na infecção de macrófagos.. Tese (Doutorado) - Instituto de Ciências Biomédicas. Universidade de São Paulo (USP). São Paulo. (02/11331-3)
NASSAR, Maira Natali. Análise estrutural e funcional da região promotora de rDNA de Leishmania (Viannia) braziliensis e estudo comparativo com as regiões de Leishmania (Leishmania). Dissertação (Mestrado) - Instituto de Biociências. Universidade de São Paulo (USP). São Paulo. (05/57809-0)