Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)

Author(s)

Baldassini, Welder Angelo ^[1] ; Braga, Camila Pereira ^[2] ; Loyola Chardulo, Luis Artur ^[1] ; Ii Vasconcelos Silva, Josineudson Augusto ^[1] ; Malheiros, Jessica Moraes ^[3] ; de Albuquerque, Lucia Galvao ^[3] ; Fernandes, Talita Tanaka ^[2] ; Padilha, Pedro de Magalhaes ^[2]

Affiliation

^[1] Sao Paulo State Univ UNESP, Coll Vet & Anim Sci, Dept Anim Breeding & Nutr, BR-18618970 Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Coll Agr & Vet Sci, Dept Anim Sci, BR-18618970 Sao Paulo - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 169, p. 65-72, FEB 15 2015.
• Web of Science Citations: 12

Abstract

The work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence (SR-XRF) and the characterisation of proteins by electrospray ionisation mass spectrometry. Forty (40) specimens were selected and divided into two experimental groups: animals with tough meat (TO) and animals with tender meat (TE). A third group (P) of Piedmontese breed animals (Bos taurus) was included to serve as a comparative model for the level of meat tenderness. The procedures were efficient and preserved the metal protein structure, enabling calcium detection in protein spots by SR-XRF at a given molecular weight range of 14-97 kDa. Two proteins (pyruvate kinase and albumin) were inferred to be related to the phenotypical differences in animals from the different groups. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 11/14850-0 - Proteins characterization µ-calpain, m-calpain and calpastatin and their relation with the quality characteristics of Nelore (Bos indicus) selected for production meat.
• Grantee: Welder Angelo Baldassini
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 09/16118-5 - Genomic tools to genetic improvement of direct economic important traits in Nelore cattle
• Grantee: Lucia Galvão de Albuquerque
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 10/51332-5 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
• Grantee: Pedro de Magalhães Padilha
• Support type: Regular Research Grants