Low-level Laser Therapy Associated With High Intensity Resistance Training on Cardiac Autonomic Control of Heart Rate and Skeletal Muscle Remodeling in Wistar Rats

Author(s)

Paolillo, Fernanda Rossi ^[1] ; Ross, Arena ^{[2, 3]} ; Dutra, Daniela Bassi ^[4] ; Marqueti Durigan, Rita de Cassia ^[5] ; de Araujo, Heloisa Selistre ^[6] ; Dutra de Souza, Hugo Celso ^[7] ; Parizotto, Nivaldo Antonio ^[8] ; Cipriano, Jr., Gerson ^[5] ; Chiappa, Gaspar ^{[9, 10, 11]} ; Borghi-Silva, Audrey ^[4]

Affiliation:Show less -

^[1] Univ Sao Paulo, Opt Grp, Phys Inst Sao Carlos IFSC, BR-05508 Sao Paulo - Brazil

^[2] Univ Illinois, Coll Appl Hlth Sci, Dept Phys Therapy, Chicago, IL - USA

^[3] Univ Illinois, Coll Appl Hlth Sci, Integrat Physiol Lab, Chicago, IL - USA

^[4] Fed Univ Sao Carlos UFSCar, Cardiopulm Physiotherapy Lab, Dept Phys Therapy, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Brasilia UNB, Fac Ceilandia, Brasilia, DF - Brazil

^[6] Fed Univ Sao Carlos UFSCar, Dept Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil

^[7] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, BR-05508 Sao Paulo - Brazil

^[8] Fed Univ Sao Carlos UFSCar, Electrothermophototherapy Lab, Dept Phys Therapy, BR-13565905 Sao Carlos, SP - Brazil

^[9] Coll Serra Gaucha, Div Phys Therapy, Sao Pelegrino - Brazil

^[10] Hosp Clin Porto Alegre, Exercise Pathophysiol Res Lab, Porto Alegre, RS - Brazil

^[11] Hosp Clin Porto Alegre, Div Cardiol, Porto Alegre, RS - Brazil

• Document type: Journal article
• Source: Lasers in Surgery and Medicine; v. 46, n. 10, p. 796-803, DEC 2014.
• Web of Science Citations: 8

Abstract

Background and ObjectivePhototherapy plus dynamic exercise can enhance physical performance and improve health. The aim of our study was to evaluate the effect of low-level laser therapy (LLLT) associated with high intensity resistance training (HIT) on cardiac autonomic and muscle metabolic responses in rats. Study Design/Materials and MethodsForty Wistar rats were randomized into 4 groups: sedentary control (CG), HIT, LLLT and HIT+LLLT. HIT was performed 3 times/week for 8 weeks with loads attached to the tail of the animal. The load was gradually increased by 10% of body mass until reaching a maximal overload. For LLLT, irradiation parameters applied to the tibialis anterior (TA) muscle were as follows: infrared laser (780nm), power of 15mW for 10 seconds, leading to an irradiance of 37.5mW/cm(2), energy of 0.15J per point and fluency of 3.8J/cm(2). Blood lactate (BL), matrix metalloproteinase gelatinase A (MMP-2) gene expression and heart rate variability (HRV) indices were performed. ResultsBL significantly increased after 8-weeks for HIT, LLLT and HIT+LLLT groups. However, peak lactate when normalized by maximal load was significantly reduced for both HIT and HIT+LLLT groups (P<0.05). MMP-2 in the active form was significantly increased after HIT, LLLT and HIT+LLLT compared tom the CG (P<0.05). There was a significant reduction in low frequency {[}LF (ms(2))] and increase in high frequency {[}HF (un)] and HF (ms(2))] for the HIT, LLLT and HIT+LLLT groups compared with the CG (P<0.05). However, the LF/HF ratio was further reduced in the LLLT and HIT+LLLT groups compared to the CG and HIT group (P<0.05). ConclusionThese results provide evidence for the positive benefits of LLLT and HIT with respect to enhanced muscle metabolic and cardiac autonomic function in Wistar rats. Lasers Surg. Med. 46:796-803, 2014. (c) 2014 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 09/01842-0 - Analysis of central and peripheral muscle metabolism and adjustments on dynamic versus resistance exercise in coronary arterial disease patients: effects of resistance physical training program based on anaerobic threshold by different methodologies
• Grantee: Audrey Borghi Silva
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/14001-9 - FLUORESCENCE SPECTROSCOPY OF TOOTH IN OVARIECTOMIZED RATS FOR OSTEOPOROSIS DIAGNOSIS
• Grantee: Fernanda Rossi Paolillo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral