Electroanalytical Determination of Imipramine in Reconstituted Serum with a Graphite-Polyurethane Composite Electrode

Author(s)

de Toledo, R. A. ^{[1, 2]} ; Santos, M. C. ^[3] ; Shim, H. ^[1] ; Mazo, L. H. ^[2]

Affiliation

^[1] Univ Macau, Fac Sci & Technol, Dept Civil & Environm Engn, Taipa, Macau Sar - Peoples R China

^[2] Inst Quim Sao Carlos, Dept Quim Fis, Grp Mat Eletroquim & Metodos Eletroanalit, BR-13560970 Sao Paulo - Brazil

^[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09020170 Santo Andre, SP - Brazil

• Document type: Journal article
• Source: International Journal of Electrochemical Science; v. 10, n. 9, p. 6975-6985, SEP 2015.
• Web of Science Citations: 5

Abstract

An electroanalytical procedure for the imipramine quantitation in commercial formulation and reconstituted serum was developed in Britton Robinson buffer solution, as supporting electrolyte (0.1 mol L-1, pH 7.0), with a GPU (graphite-polyurethane composite) electrode and the square wave voltammetry (SWV) technique. The obtained detection limit (LD) and the lower level of quantitation (LLOQ) were 58.3 ng L-1 and 84 mu g L-1, respectively. The procedure is simple, rapid, and precise for the analysis of imipramine in pharmaceutical formulation (98.9 +/- 01.3%, 24.7 +/- 0.4 mg) without the interference of the excipients. The proposal of direct analysis of imipramine in synthetic serum sample is satisfactory (90.3 +/- 3.6%, 22.6 +/- 0.9 mg, LD 26.4 mu g L-1, LLOQ 100 mu g L-1), considering the analysis was taken without prior extraction or other sample treatment, which can save costs and reduces the possibility of contamination caused by sample manipulation. (AU)

• FAPESP's process: 01/14629-0 - Electrochemical study and development of new electroanalytical methodologies for the determination of tricyclic antidepressants and neurotransmitters
• Grantee: Renata Alves de Toledo
• Support Opportunities: Scholarships in Brazil - Doctorate