Exploring novel strategies for depolymerization of plant cell-wall polysaccharides...
| Full text | |
| Author(s): Show less - |
Liberato, Marcelo V.
[1]
;
Silveira, Rodrigo L.
[2]
;
Prates, Erica T.
[2]
;
de Araujo, Evandro A.
[1]
;
Pellegrini, Vanessa O. A.
[1]
;
Camilo, Cesar M.
[1]
;
Kadowaki, Marco A.
[1]
;
Neto, Mario de O.
[3]
;
Popov, Alexander
[4]
;
Skaf, Munir S.
[2]
;
Polikarpov, Igor
[1]
Total Authors: 11
|
| Affiliation: | [1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Inst Chem, BR-13084862 Sao Paulo - Brazil
[3] State Univ Sao Paulo, Inst Biosci, BR-18618970 Sao Paulo - Brazil
[4] European Synchrotron Radiat Facil, CS40220, Grenoble - France
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 6, APR 1 2016. |
| Web of Science Citations: | 9 |
| Abstract | |
Glycoside hydrolases (GHs) play fundamental roles in the decomposition of lignocellulosic biomaterials. Here, we report the full-length structure of a cellulase from Bacillus licheniformis (BlCel5B), a member of the GH5 subfamily 4 that is entirely dependent on its two ancillary modules (Ig-like module and CBM46) for catalytic activity. Using X-ray crystallography, small-angle X-ray scattering and molecular dynamics simulations, we propose that the C-terminal CBM46 caps the distal N-terminal catalytic domain (CD) to establish a fully functional active site via a combination of large-scale multidomain conformational selection and induced-fit mechanisms. The Ig-like module is pivoting the packing and unpacking motions of CBM46 relative to CD in the assembly of the binding subsite. This is the first example of a multidomain GH relying on large amplitude motions of the CBM46 for assembly of the catalytically competent form of the enzyme. (AU) | |
| FAPESP's process: | 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis |
| Grantee: | Igor Polikarpov |
| Support type: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 14/10448-1 - Molecular aspects of plant cell wall architecture |
| Grantee: | Rodrigo Leandro Silveira |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 13/08293-7 - CCES - Center for Computational Engineering and Sciences |
| Grantee: | Munir Salomao Skaf |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 10/18773-8 - Molecular cloning, expression, purification and structural characterization of endoglucanase from Trichoderma harzianum aiming the development of enzymatic blends for production of lignocellulosic ethanol |
| Grantee: | Vanessa de Oliveira Arnoldi Pellegrini |
| Support type: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 13/15582-5 - Multiscale molecular dynamics of glycoside hydrolases and lignocellulosic substrates |
| Grantee: | Érica Teixeira Prates |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 09/52840-7 - Center of Biological and Industrial Processes for Biofuels - CeProBIO |
| Grantee: | Igor Polikarpov |
| Support type: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |