| Full text | |
| Author(s): |
Ricci, R.
;
Leite, N. C. S.
;
da-Silva, N. S.
;
Pacheco-Soares, C.
;
Canevari, R. A.
;
Marciano, F. R.
;
Webster, T. J.
;
Lobo, A. O.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Materials Science & Engineering C-Materials for Biological Applications; v. 78, p. 341-348, SEP 1 2017. |
| Web of Science Citations: | 15 |
| Abstract | |
Graphene oxide nanoribbons (O-GNR) surges as an interesting nanomaterial for biomedical applications due to feasibility to incorporate functional groups and possible bactericidal properties. Herein, high concentrations of O-GNR were biologically evaluated using human osteoblast cells and gram positive and negative bacteria. Briefly, our goal were to evaluate: (1) synthetic pathway, (2) characterization and (3) effects of O-GNR composition and structural factors as a new approach for biomedical applications. For this, O-GNR were produced combining chemical vapor deposition and oxygen plasma treatment of multiwalled carbon nanotubes. Then, we analyzed the bioactivity, cell viability, osteogenic differentiation, matrix mineralization, mRNA levels of the five genes related direct to bone repair and bactericidal effect of high concentrations of O-GNR (10 mu g mL(-1), 100 mu g mL(-1), 200 mu g mL(-1) and 300 mu g mL(-1)). Impressively, O-GNR showed no cytotoxic effects up to a concentration of 100 mu g mL(-1) and no gene expression alteration when used in its dose. We also observed that S. aureus and E. coli bacteria are susceptible to damage when incubated with 100 mu g ml(-1) of O-GNR, showing approximately 50% of bacterial death. We consider that O-GNR displays attractive properties when used at a suitable dose, displaying bactericidal effect and apparently lacking to cause damages in the bone repair process. (C) 2017 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration |
| Grantee: | Anderson de Oliveira Lobo |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications |
| Grantee: | Fernanda Roberta Marciano |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/14464-9 - Molecular and biochemical characterization of thyroid carcinomas for clinical diagnosis |
| Grantee: | Renata de Azevedo Canevari |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/20054-8 - Studies of adhesion, proliferation and cell death in the interaction of cells with nanostructured materials |
| Grantee: | Newton Soares da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/09697-0 - Electrospinning of novel functional nanobiomaterials based on peptides and bottlebrush polymers for tissue engineering |
| Grantee: | Anderson de Oliveira Lobo |
| Support Opportunities: | Scholarships abroad - Research |
| FAPESP's process: | 09/18440-1 - Development of molecular markers related to cellular resistance after photodynamic therapy in breast carcinomas |
| Grantee: | Renata de Azevedo Canevari |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/00575-1 - Functional biomembranes based on peptides and bottlebrush polymers to tissue engineering |
| Grantee: | Fernanda Roberta Marciano |
| Support Opportunities: | Scholarships abroad - Research |