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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Graphene oxide nanoribbons as nanomaterial for bone regeneration: Effects on cytotoxicity, gene expression and bactericidal effect

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Author(s):
Ricci, R. ; Leite, N. C. S. ; da-Silva, N. S. ; Pacheco-Soares, C. ; Canevari, R. A. ; Marciano, F. R. ; Webster, T. J. ; Lobo, A. O.
Total Authors: 8
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 78, p. 341-348, SEP 1 2017.
Web of Science Citations: 15
Abstract

Graphene oxide nanoribbons (O-GNR) surges as an interesting nanomaterial for biomedical applications due to feasibility to incorporate functional groups and possible bactericidal properties. Herein, high concentrations of O-GNR were biologically evaluated using human osteoblast cells and gram positive and negative bacteria. Briefly, our goal were to evaluate: (1) synthetic pathway, (2) characterization and (3) effects of O-GNR composition and structural factors as a new approach for biomedical applications. For this, O-GNR were produced combining chemical vapor deposition and oxygen plasma treatment of multiwalled carbon nanotubes. Then, we analyzed the bioactivity, cell viability, osteogenic differentiation, matrix mineralization, mRNA levels of the five genes related direct to bone repair and bactericidal effect of high concentrations of O-GNR (10 mu g mL(-1), 100 mu g mL(-1), 200 mu g mL(-1) and 300 mu g mL(-1)). Impressively, O-GNR showed no cytotoxic effects up to a concentration of 100 mu g mL(-1) and no gene expression alteration when used in its dose. We also observed that S. aureus and E. coli bacteria are susceptible to damage when incubated with 100 mu g ml(-1) of O-GNR, showing approximately 50% of bacterial death. We consider that O-GNR displays attractive properties when used at a suitable dose, displaying bactericidal effect and apparently lacking to cause damages in the bone repair process. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/18440-1 - Development of molecular markers related to cellular resistance after photodynamic therapy in breast carcinomas
Grantee:Renata de Azevedo Canevari
Support type: Regular Research Grants
FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/14464-9 - Molecular and biochemical characterization of thyroid carcinomas for clinical diagnosis
Grantee:Renata de Azevedo Canevari
Support type: Regular Research Grants
FAPESP's process: 15/09697-0 - Electrospinning of novel functional nanobiomaterials based on peptides and bottlebrush polymers for tissue engineering
Grantee:Anderson de Oliveira Lobo
Support type: Scholarships abroad - Research
FAPESP's process: 13/20054-8 - Studies of adhesion, proliferation and cell death in the interaction of cells with nanostructured materials
Grantee:Newton Soares da Silva
Support type: Regular Research Grants
FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 16/00575-1 - Functional biomembranes based on peptides and bottlebrush polymers to tissue engineering
Grantee:Fernanda Roberta Marciano
Support type: Scholarships abroad - Research