Gallicacid/hydroxypropyl-beta-cyclodextrin complex: Improving solubility for application on in vitro/in vivo Candida albicans biofilms

Author(s)

Teodoro, Guilherme Rodrigues ; Lacerda Gontijo, Aline Vidal ; Borges, Aline Chiodi ; Tanaka, Marcia Hiromi ; Gouvea Lima, Gabriela de Morais ; Salvador, Marcos Jose ; Koga-Ito, Cristiane Yumi

• Document type: Journal article
• Source: PLoS One; v. 12, n. 7 JUL 11 2017.
• Web of Science Citations: 5

Abstract

The aim of this study was to increase the solubility of gallic acid (GA) for the treatment of Candida albicans biofilm, which is very difficult to treat and requires high drug concentrations. Cyclodextrins (CDs) were used for this purpose. Complexes were evaluated by phase-solubility studies, prepared by spray drying and characterized by drug loading, scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The complexes were tested on C. albicans biofilm using in vitro and in vivo models. HP beta CD formed soluble inclusion complexes with GA. The percentage of GA in GA/HP beta CD was 10.8 +/- 0.01%. The SEM and DSC analyses confirmed the formation of inclusion complexes. GA/HP beta CD maintained the antimicrobial activity of the pure GA. GA/HP beta CD was effective on C. albicans biofilms of 24 and 48h. The in vivo results showed an anti-inflammatory activity of GA/HP beta CD with no difference in invading hypha counting among the groups. This study encourages the development of new antifungal agents. (AU)

• FAPESP's process: 12/16805-5 - ANTIFUNGAL ACTIVITY OF PHENOLYIC ENRICHED FRACTION OF Buchenavia tomentosa AND SOME ISOLATED SUBSTANCES BEFORE AND AFTER ENCAPSULATION WITH BETA-CYCLODEXTRIN
• Grantee: Guilherme Rodrigues Teodoro
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/00037-1 - Antifungal activity of phenolic enriched fraction of Buchenavia tomentosa and some isolated substances before and after encapsulation in Beta-cyclodextrin
• Grantee: CRISTIANE YUMI KOGA ITO
• Support type: Regular Research Grants
• FAPESP's process: 14/02354-7 - Antifungal activity and genotoxicity of atmospheric pressure nonthermal plasma jet
• Grantee: Aline Chiodi Borges
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/03470-3 - Aplications of low temperature atmospheric pressure plasma in dentistry
• Grantee: Gabriela de Morais Gouvêa Lima
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 14/09666-4 - DEPLOYMENT OF ASSAYS FOR EVALUATION OF BIOPHARMACEUTIC CHARACTERISTICS AND BIOACTIVITY OF COMPOUNDS AND BIOACTIVE PRODUCTS USING IN VITRO MODELS OF INVASIVE CANDIDIASIS
• Grantee: Aline Vidal Lacerda Gontijo
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 14/13469-0 - Effects of treatment with hydroxyurea in the oral cavity of pediatric patients with sickle cell anemia
• Grantee: Marcia Hiromi Tanaka
• Support type: Scholarships in Brazil - Post-Doctorate