Topical photochemical nitric oxide release from porous poly(vinyl alcohol) membrane for visible light modulation of dermal vasodilation

Author(s)

Lourenco, Sarah De M. ; de Oliveira, Marcelo G.

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY; v. 346, p. 548-558, SEP 1 2017.
• Web of Science Citations: 5

Abstract

Complications of functional peripheral artery disease (PAD), like chronic wounds and Raynaud's disease and phenomenon, have stimulated the development of topical nitric oxide (NO)-releasing biomaterials capable of restoring the blood flow locally. In this work, we used phase inversion technique to prepare porous membranes of poly(vinyl alcohol) (PVA) functionalized with NO -releasing S-nitrosothiol (SNO) moieties. This strategy led to crosslinked amorphous membranes with swelling degree of 750 wt% and interconnected macropores with diameters greater than 600 mu m. Controlling the extent of the Snitrosation reaction allowed obtaining PVA-SNO membranes that spontaneously release NO in the range of 33-53 nmol/cm(2) in the dark. Irradiation of these membranes with visible light, led to first order NO release kinetics with rate constant of (5.6 +/- 0.2) x 10(-3)s(-1) and increased the amount of NO released to 64-101 nmol/cm(2). Topical application of PVA-SNO membrane for 4 min on the healthy skin, led to a fast and localized 300% increase in the dermal blood flow, which was enhanced to 900% when the membrane was irradiated with visible light, during the same period. These results show that the photochemical NO release from macroporous PVA-SNO membrane is an effective tool for modulating NO actions in the treatment/prevention of PAD symptoms and other biomedical applications. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release
• Grantee: Marcelo Ganzarolli de Oliveira
• Support Opportunities: Research Projects - Thematic Grants