Validation of spectrophotometric microplate methods for polyphenol oxidase and peroxidase activities analysis in fruits and vegetables

Author(s)

Siguemoto, Erica Sayuri ^[1] ; Wilhelms Gut, Jorge Andrey ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Escola Politecn, Dept Chem Engn, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Food Res Ctr FoRC, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: FOOD SCIENCE AND TECHNOLOGY; v. 37, n. 1, p. 148-153, DEC 2017.
• Web of Science Citations: 2

Abstract

Enzymes polyphenol oxidase (PPO) and peroxidase (POD) play important roles in the processing of fruits and vegetables, since they can produce undesirable changes in color, texture and flavor. Classical methods of activity assessment are based on cuvette spectrophotometric readings. This work aims to propose, to validate and to test microplate spectrophotometric methods. Samples of apple juice and lyophilized enzymes from mushroom and horseradish were analyzed by the cuvette and microplate methods and it was possible to validate the microplate assays with satisfactory results regarding linearity, repeatability, accuracy along with quantitation and detection limits. The proposed microplate methods proved to be reliable and reproducible as the classical methods besides having the advantages of allowing simultaneous analysis and requiring a reduced amount of samples and reactants, which can beneficial to the study of enzyme inactivation in the processing of fruits and vegetables. (AU)

• FAPESP's process: 14/17534-0 - Evaluation of the effects of focused microwave processing on liquid foods
• Grantee: Jorge Andrey Wilhelms Gut
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/06026-4 - Study of continuous-flow microwave thermal processing of cloudy apple juice
• Grantee: Érica Sayuri Siguemoto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC