| Grant number: | 14/06026-4 |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| Start date: | July 01, 2014 |
| End date: | February 28, 2018 |
| Field of knowledge: | Agronomical Sciences - Food Science and Technology - Food Engineering |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Jorge Andrey Wilhelms Gut |
| Grantee: | Érica Sayuri Siguemoto |
| Host Institution: | Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 13/07914-8 - FoRC - Food Research Center, AP.CEPID |
| Associated scholarship(s): | 15/11677-7 - Inactivation kinetics of foodborne pathogens in cloudy apple juice by microwave and conventional thermal processing, BE.EP.DR |
Abstract Introduction: Focused microwave is a technology with wide potential to continuous-flow pasteurization of fluid foods, since promotes rapid and uniform heating avoiding losses in nutritional quality. Objective: To analyze the continuous-flow microwave thermal processing of cloudy apple juice, taking into account enzyme inactivation and nutritional quality. Methods: The cloudy apple juice will be characterized through physicochemical analysis, such as pH, titratable acidity, soluble solids, color and turbidity. The inactivation kinetics of enzymes polyphenol oxidase (PPO) and pectin esterase (PE) will be assessed using discontinuous microwave treatment and conventional treatment. According to the results, the cloudy apple juice will be processed in a continuous-flow microwave system and will be compared with continuous-flow conventional system. The samples will be lyophilized for posterior analysis: physicochemical characterization, organic acids by High Performance Liquid Chromatography with diode array detector (HPLC/DAD), ascorbic acid by HPLC/DAD, total phenolic compounds by Folin-Ciocalteau method, phenolic profile will be characterized by HPLC/DAD coupled to mass spectrometry with electrospray ionization system, and antioxidant activities (ORAC and DPPH). Appropriate statistical test will be used to determine significant differences between the thermal systems. | |
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