Functional and proteomic comparison of Bothrops jararaca venom from captive specimens and the Brazilian Bothropic Reference Venom

Author(s)

de Farias, Iasmim Baptista ^{[1, 2]} ; de Morais-Zani, Karen ^{[1, 2]} ; Serino-Silva, Caroline ^{[1, 2]} ; Sant'Anna, Savio S. ^[1] ; da Rocha, Marisa M. T. ^[1] ; Grego, Kathleen F. ^[1] ; Andrade-Silva, Debora ^[3] ; Serrano, Solange M. T. ^[3] ; Tanaka-Azevedo, Anita M. ^{[1, 2]}

Affiliation

^[1] Inst Butantan, Lab Herpetol, BR-05503000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Butantan, Inst Pesquisas Tecnol, Inst Ciencias Biomed, Interunidades Biotecnol, BR-05503000 Sao Paulo - Brazil

^[3] Inst Butantan, Immune Response & Cell Signaling CeTICS, Ctr Toxins, Lab Especial Toxinol Aplicada, BR-05503000 Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF PROTEOMICS; v. 174, p. 36-46, MAR 1 2018.
• Web of Science Citations: 4

Abstract

Snake venom is a variable phenotypic trait, whose plasticity and evolution are critical for effective antivenom production. A significant reduction of the number of snake donations to Butantan Institute (Sao Paulo, Brazil) occurred in recent years, and this fact may impair the production of the Brazilian Bothropic Reference Venom (BBRV). Nevertheless, in the last decades a high number of Bothrops jararaca specimens have been raised in captivity in the Laboratory of Herpetology of Butantan Institute. Considering these facts, we compared the biochemical and biological profiles of B. jararaca venom from captive specimens and BBRV in order to understand the potential effects of snake captivity upon the venom composition. Electrophoretic analysis and proteomic profiling revealed few differences in venom protein bands and some differentially abundant toxins. Comparison of enzymatic activities showed minor differences between the two venoms. Similar cross-reactivity recognition pattern of both venoms by the antibothropic antivenom produced by Butantan Institute was observed. Lethality and neutralization of lethality for B. jararaca venom from captive specimens and BBRV showed similar values. Considering these results we suggest that the inclusion of B. jararaca venom from captive specimens in the composition of BBRV would not interfere with the quality of this reference venom. (AU)

• FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
• Grantee: Hugo Aguirre Armelin
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 16/16935-7 - A post-translational modification perspective of Bothrops venom toxins: site-specific proteomic characterization of N-glycans and Cys residues.
• Grantee: Débora Andrade Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/11108-0 - Comparative study of biomolecules composition of Bothrops jararaca and Crotalus durissus terrificus venom born and maintained at the Butantan Institute and newly arrived from nature
• Grantee: Karen de Morais Zani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/01890-0 - Comparative study of Crotalus durissus terrificus snake venoms born in captivity in the Laboratory of Herpetology of Butantan Institute and the national crotalic reference venom
• Grantee: Anita Mitico Tanaka-Azevedo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/16908-2 - Proteomic characterization and enzymatic and pathophysiological activities of the venom of the snake species that compose the Bothrops neuwiedi group
• Grantee: Karen de Morais Zani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/05357-4 - Comparative study of reference Bothrops venom in relation to the venom of Bothrops jararaca born in captivity in herpetology laboratory of the Instituto Butantan
• Grantee: Anita Mitico Tanaka-Azevedo
• Support Opportunities: Regular Research Grants